Sample Holder Internal Reflection for Multi-Directional Bioimaging
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Solution Overview
Problem
Current methods for analyzing biological samples are limited in providing efficient and accurate optical and image analysis, particularly for small samples and diverse biological components, as they often rely on traditional illumination techniques that do not effectively utilize internal reflections for comprehensive examination.
Innovation Solution
The development of a sample holder system that incorporates optically transmissive materials with reflective surfaces, enabling both epi-illumination and trans-illumination through partial or total internal reflection, allowing for simultaneous illumination from multiple directions and enhancing the detection of cellular components and structures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a single light source is used on one side of the sample holder, then the device complexity is reduced, but the illumination quality and measurement precision are insufficient
Solution Approach 1:
The illumination system is segmented into two distinct illumination modes: epi-illumination (light incident from above) and trans-illumination (light incident from below). This segmentation allows the system to provide comprehensive optical examination from multiple directions using a single light source positioned on one side of the sample holder, resolving the contradiction by dividing the illumination function into spatially distinct paths rather than requiring multiple light sources.
Solution Approach 2:
The patent utilizes the third dimension (vertical depth) by incorporating a sample holder with specific geometric features that redirect light paths. The light source on one side generates both downward-propagating light (for epi-illumination) and laterally redirected light (for trans-illumination), effectively creating multi-directional illumination from a single source position through spatial dimensionality.
2Adaptability or versatility
If multiple illumination directions are provided, then the optical analysis capability is improved, but the device complexity increases
Solution Approach 1:
The sample holder is designed with multi-functional geometric features that serve dual purposes: structural support and optical path control. The holder's configuration enables it to guide light for both epi-illumination and trans-illumination modes simultaneously, making the illumination system universal and adaptable to different examination requirements without increasing overall device complexity.
Solution Approach 2:
The sample holder structure itself performs the function of light path management. By incorporating reflective surfaces and specific geometric configurations into the holder, the system uses the holder's own structure to redirect and distribute light, eliminating the need for separate optical components and reducing system complexity while maintaining versatile illumination capabilities.
3Use of energy by moving object
If internal reflection is utilized for light distribution, then the illumination efficiency is improved, but the manufacturing precision requirements increase
Solution Approach 1:
The patent employs parameter changes in the form of surface treatment and geometric configuration of the sample holder. By modifying the surface properties (creating reflective surfaces) and geometric parameters (angles, shapes) of the holder, the system optimizes internal reflection to enhance light distribution efficiency. These parameter adjustments are designed to be achievable within standard manufacturing tolerances, balancing optical performance with manufacturing feasibility.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables more comprehensive and accurate analysis of biological samples by providing enhanced illumination and imaging capabilities, improving the identification and quantification of cellular features, even in small samples, and facilitating precise measurements and diagnostics.
Implementation Method 1
a sample holder having an optically transmissive portion and a portion configured to provide internal reflection of light within the sample holder
Implementation Method 2
internal reflections may include partial internal reflection and may include total internal reflection of light
Data Source
AI summary
Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.