Cryo-Microscopy Sample Holder Switching for Carrier Handling

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Solution Overview

Problem

Existing sample holder transfer devices in cryo-microscopy require manual operation to open and close the sample carrier fixing element, which complicates the process of inserting and removing sample carriers from the sample holder.

Innovation Solution

A sample holder transfer device with a switching mechanism that automates the opening and closing of the sample carrier fixing element, allowing for simplified insertion and removal of sample carriers without manual intervention.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of operation

If manual operation is used to open and close the sample carrier fixing element, then the device structure remains simple, but the operation process becomes complicated and time-consuming

Engineering Contradiction:
Improveoperation processVSAvoiddevice structure
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The sample carrier fixing element is designed to automatically open and close through its own mechanical movement. When the sample carrier is inserted or removed, the fixing element responds automatically without requiring external manual intervention, making the system serve itself.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The manual mechanical operation of opening and closing the fixing element is replaced by an automated mechanical linkage system. The switching mechanism uses mechanical advantage and linkage geometry to convert a simple switching action into the coordinated movement needed to open/close the fixing element and position the sample carrier.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If manual operation of the sample carrier fixing element is required, then the device structure remains simple, but productivity decreases due to additional manual steps

Engineering Contradiction:
Improvehandling efficiencyVSAvoidswitching mechanism
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

Multiple functions are merged into a single switching mechanism. One switching action simultaneously performs: opening the fixing element, allowing sample carrier insertion/removal, and resetting the fixing element to its closed position. This consolidation of functions into one mechanism improves productivity while controlling complexity.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The fixing element is pre-configured with spring biasing and mechanical linkages that prepare it for automatic operation. The spring pre-load and linkage geometry are designed in advance so that when the switching mechanism activates, the fixing element responds immediately without requiring additional manual adjustments or steps.

Inventive Principle:
Principle #10Preliminary action

3Reliability

If manual operation is used, then the device structure remains simple, but the risk of contamination increases due to prolonged manual handling

Engineering Contradiction:
Improvecontamination riskVSAvoidautomation mechanism
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The system minimizes human intervention by making the fixing element self-operating. The mechanical linkage and spring system allow the fixing element to open and close automatically based on sample carrier presence, reducing the time samples are exposed to potential contamination sources.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The automated switching mechanism enables rapid opening and closing of the fixing element, minimizing the duration that samples are exposed during the loading/unloading process. The quick mechanical action skips through the vulnerable period where contamination could occur.

Inventive Principle:
Principle #21Skipping (Rushing through)

Data Source

PatentEP4167013B1Sample holder transfer device
Publication Date: 2025.02.19 LEICA MIKROSYSTEME GMBH
  • EP4167013B1 patent drawingFigure 1
  • EP4167013B1 patent drawingFigure 2a
  • EP4167013B1 patent drawingFigure 2b

AI summary

The present invention relates to a sample holder transfer device (100) for use in cryo-microscopy for transferring a sample holder (150) to an analysing or processing unit, said sample holder (150) being configured for holding a sample carrier (170) carrying a sample, said sample holder transfer device (100) being configured to receive the sample holder (150), the sample holder (150) comprising a sample carrier fixing element (252), at least one section of the sample carrier fixing element (252) being configured, when in a first position, to fix the sample carrier (170) to the sample holder (150), and, when in a second position, to release the sample carrier (170) or to provide access to an area (254) where the sample carrier (170) is to be placed, said sample holder transfer device (100) further comprising a switching mechanism (110) operable to switch the at least one section of the sample carrier fixing element (252) of the sample holder (150) from the first position to the second position and/or from the second position to the first position.