Sample Plate Recesses for Multiplexed Assay Bead Retention
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Solution Overview
Problem
Conventional ELISA techniques face challenges in efficiently performing multiple tests on a single patient sample, leading to increased processing time and costs, and difficulties in quality control due to the need for multiple microplates and complex programming in automated systems.
Innovation Solution
A sample plate with a format that allows for the retention of reagent beads or microspheres in recesses or through holes, enabling a fluid-tight seal, which facilitates the simultaneous performance of multiple tests in a single well through multiplexing, reducing the footprint of automated apparatus and improving flexibility.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional ELISA techniques are used to perform multiple tests on a single patient sample, then multiple microplates are required, but this increases processing time and costs
Solution Approach 1:
The patent combines multiple test capabilities into a single microplate by incorporating multiple recesses in the base portion, each capable of retaining different reagent beads. This merging approach allows simultaneous performance of multiple tests on a single patient sample within one microplate, eliminating the need to sequentially process multiple microplates and thereby reducing processing time.
Solution Approach 2:
The patent utilizes the base portion of the microplate as an additional dimension for organizing multiple tests. By creating multiple recesses within the base portion at different positions, the system transforms a single-test platform into a multi-test platform without increasing the vertical profile or interfering with the liquid handling space above the base.
2Adaptability or versatility
If conventional ELISA techniques are used to perform multiple tests on a single patient sample, then multiple microplates are required, but this increases costs
Solution Approach 1:
The patent merges multiple test functions into a single microplate unit, reducing the total quantity of microplates required. This consolidation decreases material costs, handling costs, and waste generation while maintaining the capability to perform multiple different tests simultaneously on the same sample.
3Extent of automation
If conventional ELISA techniques are used, then automated systems require complex programming, but this reduces ease of operation
Solution Approach 1:
The patent segments the microplate base into multiple distinct recesses, each designed to retain specific reagent beads. This segmentation creates physically distinct zones that can be independently addressed by automated systems, simplifying programming by allowing each recess to be treated as a separate, identifiable location rather than requiring complex coordination of multiple microplates.
Solution Approach 2:
The recesses are designed to automatically retain reagent beads through their geometric configuration, eliminating the need for complex active retention mechanisms. This self-retaining feature simplifies automated operation by reducing the number of steps and programming required to ensure proper bead placement and retention during the assay process.
4Adaptability or versatility
If conventional ELISA techniques are used, then multiple microplates are required, but this increases device footprint
Solution Approach 1:
The patent merges multiple test capabilities into a single microplate, thereby reducing the number of microplates that need to be loaded onto the automated apparatus. This consolidation decreases the footprint required for plate loading areas, incubation zones, washing stations, and detection systems, allowing the same functionality to be achieved in a more compact configuration.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The improved sample plate format enables efficient multiplexing, reducing processing time and costs by allowing multiple tests to be performed simultaneously, enhancing the flexibility and efficiency of automated systems while maintaining quality control.
Implementation Method 1
the bead forms a substantially fluid-tight circumferential seal with a wall of the base portion which defines the recess
Data Source
AI summary
A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. A method of using the sample plate and a kit comprising the sample plate is also disclosed.