Integrated Sample Preparation Vessel with Chromatography Medium
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Solution Overview
Problem
Current sample preparation methods for bio-organic compounds involve multiple transfer steps, leading to sample loss, contamination, and increased processing time, especially when handling small sample quantities, and require extensive handling and costly single-use materials.
Innovation Solution
A sample preparation container with a reaction chamber and integrated chromatography medium that allows for lysis, purification, and enrichment of bio-organic compounds in a single vessel, eliminating the need for transfer steps and incorporating a self-sealing design to reduce contamination and enhance safety.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple transfer steps are used for sample preparation, then purification and enrichment can be achieved, but sample loss increases and processing time extends
Solution Approach 1:
The patent combines multiple sample preparation functions (lysis, purification, enrichment) into a single reaction vessel with integrated chromatography medium, eliminating the need for transferring samples between multiple vessels and thereby reducing sample loss while maintaining purification quality
Solution Approach 2:
The reaction vessel is designed to perform multiple functions simultaneously - serving as both the lysis chamber and the purification chamber with integrated chromatography medium, allowing the same vessel to handle both cell disruption and protein purification without sample transfer
2Reliability
If multiple transfer steps are used for sample preparation, then purification can be achieved, but processing time increases
Solution Approach 1:
By merging the lysis chamber and purification chamber into one integrated vessel with chromatography medium attached to the inner wall, the patent eliminates multiple transfer steps and enables simultaneous performance of lysis and purification operations, significantly reducing processing time
Solution Approach 2:
The integrated design allows continuous processing where lysis occurs in the same vessel where purification subsequently occurs, eliminating idle transfer time and maintaining continuous useful action throughout the sample preparation process
3Reliability
If multiple vessels are used for sample preparation, then purification can be achieved, but contamination risk increases
Solution Approach 1:
The patent merges multiple preparation steps into a single closed reaction vessel system, eliminating the openings and transfers between vessels that create contamination pathways, thereby reducing contamination risk while maintaining purification quality
Solution Approach 2:
The chromatography medium is extracted and attached directly to the inner wall of the reaction vessel, eliminating the need for separate purification vessels and the associated transfer operations that expose samples to contamination risks
4Reliability
If extensive handling is performed for sample preparation, then purification can be achieved, but costs increase
Solution Approach 1:
By combining multiple sample preparation functions into a single integrated reaction vessel, the patent reduces the number of consumable vessels and materials needed, thereby lowering processing costs while maintaining purification quality
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces sample loss and processing time, minimizes unwanted modifications, and decreases costs by enabling all sample preparation steps to be performed in a single vessel, facilitating automation and safer handling of hazardous materials.
Implementation Method 1
said chromatography medium is configured to purify and/or enrich said bio-organic compounds
Implementation Method 2
lysis, e.g. by sonication and/or boiling
Implementation Method 3
lysis, e.g. by sonication and/or boiling
Data Source
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AI summary
The present invention relates to sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub- components of said cellular material and/or viruses, the container comprising a reaction chamber and a chromatography medium; wherein said reaction chamber is for holding said cellular material, viruses and/or sub-components of said cellular material and/or viruses and is configured such that at least one of the following reactions can be performed therein: lyis, e.g. by sonication and/or boiling; chromatographic purification; reduction; alkylation; and enzymatic reactions such as proteolysis; wherein said chromatography medium is configured to purify and/or enrich said bio-organic compounds; wherein (a) said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds; or (b) said sample preparation container further comprises a receiving chamber for receiving said bio-organic compounds, said receiving chamber being adjacent to said chromatography medium such that said chromatography medium separates said reaction chamber from said receiving chamber, and the outer face of said receiving chamber is closed and configured to be opened for obtaining purified and/or enriched bio-organic compounds.