Integrated Sample Processing Device with Gold Nanoparticle Filter
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Solution Overview
Problem
Conventional sample processing devices for detecting antibodies or antigens are not user-friendly and require complex processing steps, making them inefficient for rapid and sensitive detection.
Innovation Solution
A sample processing device with a buffer container and filter cap coated with a colloidal gold nanoparticle-based color conjugate, allowing for easy sample preparation and rapid detection through a membrane test device with immobilized polymers for binding target antigens or antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If conventional sample processing devices are used for detecting antibodies or antigens, then detection functionality is provided, but the devices require complex processing steps and are not user-friendly
Solution Approach 1:
The invention merges the buffer container and buffer cap into a single integrated sample processing device. The buffer cap is designed to be snuggly mountable to the buffer container, combining multiple functions (buffer storage, filtration, and detection) into one device, thereby reducing operational complexity and improving ease of use
2Productivity
If conventional sample processing devices are used, then detection is possible, but rapid and sensitive detection cannot be achieved
Solution Approach 1:
The buffer cap is pre-coated with color conjugate comprising colloidal gold nanoparticles coupled to polymer before use. This preliminary preparation eliminates the need for separate reagent addition steps during operation, enabling rapid detection while maintaining high sensitivity through the pre-positioned detectable label
Solution Approach 2:
The invention utilizes color conjugate with detectable label (colloidal gold nanoparticles) that produces visible color changes upon binding to target antigens or antibodies. This allows for rapid, sensitive detection without requiring complex instrumentation, achieving both speed and precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables rapid and sensitive detection of antibodies or antigens with high specificity and low false rates, achieving results in under two minutes with a simple one-step procedure, and is suitable for various infectious diseases.
Implementation Method 1
The filter may be coated with a color conjugate comprising a color reagent coupled to a polymer. In some embodiments, the color reagent comprises colloidal gold nanoparticles.
Implementation Method 2
the test region of the membrane comprises a polymer immobilized thereon adapted to bind to a target antibody or antigen in the sample
Implementation Method 3
The mixture may then be allowed to pass through a filter coated with a color conjugate before flowing the mixture out of the buffer cap through a fluid outlet end
Data Source
AI summary
A sample testing device, a method of preparing a filter for use with the sample testing device, a kit comprising the sample testing device, and a process for detecting antibodies and/or antigens are disclosed.