Sample Reservoir Inserts for Bone Marrow Cell Separation
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Solution Overview
Problem
Current bone marrow separation methods are inefficient, prone to contamination, and unsuitable for time-sensitive applications due to their complexity, variability, and inability to process multiple samples simultaneously.
Innovation Solution
The use of single-well and multi-well sample reservoir inserts that allow for centrifugation of bone samples, facilitating the separation of bone marrow cells from bones by flushing them into a collection reservoir while minimizing damage and contamination, using a centrifuge vial system with specific opening sizes and structural members to support the sample upright.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple-step separation methods are used, then cells can be separated from bone marrow, but the process becomes complex and time-consuming
Solution Approach 1:
The device is divided into distinct functional segments: a collection reservoir for receiving bone marrow, a separation reservoir with filtration structures, and a cell collection chamber. This segmentation allows each component to perform its specific function efficiently, reducing overall process complexity while maintaining separation effectiveness
Solution Approach 2:
The invention employs a nested structure where the separation reservoir is positioned within the collection reservoir, and the cell collection chamber is integrated within the separation reservoir. This nesting allows multiple separation stages to occur simultaneously in a compact configuration, reducing method complexity without compromising separation reliability
2Quantity of substance
If traditional separation methods are used, then bone marrow cells can be obtained, but contamination with non-hematopoietic cells occurs
Solution Approach 1:
The separation reservoir incorporates filtration structures with specific pore sizes and surface properties optimized for capturing non-hematopoietic cells while allowing bone marrow cells to pass through. This localized functional differentiation enables selective cell separation, improving purity without sacrificing cell yield
Solution Approach 2:
The invention utilizes porous filtration structures within the separation reservoir that are specifically designed to retain non-hematopoietic cells based on size and surface characteristics. These porous materials enable physical filtration of contaminating cells while permitting the passage of desired bone marrow cells, thereby reducing contamination without compromising cell recovery
3Productivity
If rapid separation is performed, then time-sensitive applications are satisfied, but cell damage may increase
Solution Approach 1:
The device enables dynamic control of the separation process through adjustable centrifugal forces and fluid flow rates. By optimizing these parameters, the system achieves rapid cell separation while maintaining gentle handling conditions that prevent cell damage, thus simultaneously improving productivity and reducing cell stress
Solution Approach 2:
The invention introduces buffer solutions and protective media as intermediaries during the separation process. These intermediaries cushion mechanical stresses on cells during rapid processing, enable faster throughput through optimized fluid dynamics, and maintain cell viability throughout the accelerated separation procedure
4Productivity
If parallel sample processing is enabled, then throughput increases, but device complexity increases
Solution Approach 1:
The invention combines multiple separation chambers and filtration structures into a single integrated device that can process multiple samples simultaneously. By merging these functions into one unified system with shared components, the device achieves parallel processing capability without proportionally increasing overall complexity
Solution Approach 2:
The separation reservoir and filtration structures are designed with universal applicability to handle different bone marrow sample types and volumes. This multi-functionality allows a single device configuration to process multiple samples in parallel using the same separation principles, increasing throughput without requiring separate specialized systems for each sample type
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables rapid, standardized, and high-throughput separation of bone marrow cells with reduced contamination and damage, suitable for time-sensitive applications and large sample numbers, improving yield and processing efficiency.
Implementation Method 1
centrifuging a sample reservoir insert containing a bone sample
Data Source
AI summary
Embodiments described herein include sample reservoir insert devices and methods of using and making the same. The embodiments can be used for the separation of bone marrow cells from murine and human specimens by centrifugal force. The devices can be sterile, single use rigid embodiments with an attached cap coupled to the sample reservoir insert with a hinge within which the specimens will be placed during the separation procedure.