SAWTOOTH Lettuce Cultivar CRISPR Breeding Stability
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Solution Overview
Problem
Current lettuce cultivars lack stability and high yield, with existing breeding methods struggling to consistently produce agronomically sound and unique varieties that maximize land use and improve horticultural qualities.
Innovation Solution
Development of the novel lettuce cultivar SAWTOOTH, which includes specific physiological and morphological characteristics, along with methods for vegetative propagation, tissue culture, and introduction of desired traits through genetic modification using New Breeding Techniques, such as CRISPR/Cas system, to enhance traits like disease resistance, drought tolerance, and nutritional quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional breeding methods are used to develop lettuce cultivars, then variety diversity can be achieved, but stability and consistency of agronomic qualities cannot be ensured
Solution Approach 1:
The patent applies parameter changes by modifying specific genetic parameters through CRISPR/Cas9 genome editing to achieve desired agronomic traits. By precisely altering genetic parameters rather than relying on conventional crossing, the invention achieves both variety diversity and stability of agronomic qualities simultaneously.
Solution Approach 2:
The patent replaces the mechanical system of conventional breeding (physical crossing and selection) with a molecular-level approach using CRISPR/Cas9 genome editing. This substitution enables precise control over genetic traits, ensuring both diversity and stability without the randomness inherent in conventional methods.
2Productivity
If conventional breeding methods are used, then some agronomic improvements can be achieved, but high yield and superior horticultural qualities cannot be consistently produced
Solution Approach 1:
The patent replaces conventional mechanical breeding with CRISPR/Cas9 genome editing to consistently produce high-yielding lettuce with superior horticultural qualities. This molecular approach ensures reliable expression of desired traits without the variability inherent in conventional breeding.
Solution Approach 2:
The patent segments the breeding process into specific target gene modifications using CRISPR/Cas9, allowing independent optimization of yield-related traits and horticultural qualities. This segmentation enables consistent production of superior varieties by addressing each trait separately through precise genome editing.
3Productivity
If land use is maximized through intensive breeding, then yield increases, but plant agronomic and horticultural qualities may deteriorate
Solution Approach 1:
The patent uses parameter changes through CRISPR/Cas9 editing to improve yield-related parameters without compromising agronomic and horticultural qualities. By precisely modifying specific genes rather than selecting from broad populations, the invention achieves land use efficiency while maintaining superior plant qualities.
Data Source
AI summary
Novel lettuce, such as lettuce designated SAWTOOTH is disclosed. In some embodiments, the disclosure relates to the seeds of lettuce designated SAWTOOTH, to the plants and plant parts of lettuce designated SAWTOOTH, and to methods for producing a lettuce plant by crossing the lettuce designated SAWTOOTH with itself or another lettuce plant. The disclosure further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other lettuce plants derived from the lettuce designated SAWTOOTH.