Garden Bean SB4348 Breeding via Marker-Assisted Selection

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Solution Overview

Problem

The development of new garden bean cultivars is a time-consuming and unpredictable process due to the complexity of inheritance and the lack of control over genetic traits, making it difficult to identify and produce superior cultivars with desirable traits such as high yield, disease resistance, and adaptability.

Innovation Solution

The development of the novel garden bean cultivar SB4348, which is a bush snap bean suitable for the fresh market, combining traits like high yield, disease resistance, and adaptability through a combination of traditional breeding methods and genetic engineering, allowing for the introduction of transgenes for traits like herbicide resistance and improved nutritional quality.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop new garden bean cultivars, then genetic diversity and adaptability are improved, but the development time and unpredictability increase significantly

Engineering Contradiction:
ImproveadaptabilityVSAvoiddevelopment time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (manual crossing, selection, and evaluation) with molecular marker-assisted selection technology. This substitution allows for direct detection of desired genetic traits at the DNA level, dramatically reducing the time required to identify superior cultivars while maintaining genetic diversity and adaptability through controlled crossing programs.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent introduces molecular markers as intermediaries between phenotypic observation and genotypic selection. These markers serve as reliable indicators of desired traits, allowing breeders to select plants based on genetic composition rather than waiting for phenotypic expression, thereby accelerating cultivar development without compromising adaptability.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If conventional breeding procedures are used without DNA level control, then natural genetic combinations are generated, but the ability to predict and control final resulting lines is lost

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidgenetic trait control
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent replaces random phenotypic selection with molecular marker-assisted selection, substituting the mechanical process of growing and evaluating plants with a precise molecular detection system. This allows for controlled selection of specific genetic combinations, improving both breeding efficiency and genetic trait control simultaneously.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent implements feedback loops where molecular marker data continuously inform breeding decisions. By monitoring genetic composition at each generation, breeders can adjust crossing strategies to achieve desired trait combinations, creating a controlled yet diverse breeding program that improves both productivity and precision.

Inventive Principle:
Principle #23Feedback

3Measurement precision

If replicated observations are used to estimate genetic worth, then measurement accuracy is improved, but the complexity and time required for evaluation increases

Engineering Contradiction:
Improvegenetic worth estimation accuracyVSAvoidevaluation process complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent replaces complex multi-location field trials and replicated phenotypic observations with molecular marker analysis. This substitution provides accurate genetic worth estimation through direct DNA detection, eliminating the need for complex evaluation protocols while maintaining or improving measurement precision.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent performs genetic evaluation at the DNA level before phenotypic expression occurs. By detecting desired traits through molecular markers in early plant stages, the need for replicated field observations is eliminated, simplifying the evaluation process while maintaining accuracy through direct genetic detection.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS8049076B2Garden bean SB4348
Publication Date: 2011.11.01 SYNGENTA CROP PROTECITON AG
  • US8049076B2 patent drawing

AI summary

A novel garden bean cultivar, designated SB4348, is disclosed. The invention relates to the seeds of garden bean cultivar SB4348, to the plants of garden bean line SB4348 and to methods for producing a garden bean plant by crossing the cultivar SB4348 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4348.