Scattered Light Ratio for Foreign Body Reaction Detection
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Solution Overview
Problem
The method for measuring scattered light in latex immunoassays is susceptible to foreign-body reactions such as air bubbles and nonspecific agglutination, making it difficult to distinguish between normal aggregates and those caused by foreign matter, leading to increased noise in measurement results.
Innovation Solution
An automatic analysis device with two light receivers positioned at different angles to measure scattered light, allowing for the calculation of a ratio that differentiates between latex particle aggregates and foreign matter, thereby determining if the measurement results are influenced by foreign-body reactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If scattered light measurement is used to increase sensitivity in latex immunoassay, then measurement sensitivity is improved, but foreign-body reactions (air bubbles, nonspecific agglutination) cause increased noise and false readings
Solution Approach 1:
The scattered light measurement is segmented by using multiple light receivers positioned at different angles (e.g., 90° and 45°) to capture scattered light from different directions. By comparing the ratio of scattered light intensities at these different angles, the system can distinguish between specific agglutination reactions (latex particles) and foreign-body reactions (air bubbles, dust), thereby maintaining high sensitivity while improving measurement reliability
Solution Approach 2:
The invention changes the measurement parameter from single-angle scattered light intensity to the ratio of scattered light intensities at multiple angles. This parameter transformation enables differentiation between specific and non-specific reactions, as latex particle aggregates and foreign matter produce different angular scattering patterns, thus resolving the contradiction between sensitivity and reliability
2Device complexity
If single-angle scattered light measurement is used, then device complexity is low, but inability to distinguish foreign matter leads to increased measurement noise
Solution Approach 1:
The invention adds another dimension to the measurement by introducing multiple light receivers at different angular positions. Instead of measuring only the intensity of scattered light at a single angle, the system measures the angular distribution of scattered light by comparing intensities at multiple angles, thereby enabling differentiation between specific and non-specific reactions with relatively simple additional hardware
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate determination of whether foreign-body reactions are affecting the measurement results, allowing for high-accuracy quantification of substance concentration using scattered light measurement methods.
Implementation Method 1
a first light receiver 161 that receives scattered light 21a in a first direction, which is a direction away from the optical axis of the irradiation light 18 or the transmitted light 19 by a first angle in the air, and a second light receiver 162 that receives scattered light 21b in a second direction, which is a direction away from the optical axis of the irradiation light 18 or the transmitted light 19 by a second angle in the air
Data Source
Figure 1
Figure 2~3
Figure 4
AI summary
To enable determination of if there is an influence of foreign-body reactions on the result of quantitative determination conducted with a scattered light measurement method. Proposed is an automatic analysis device including a light source configured to irradiate a reaction solution with light, a plurality of light receivers configured to receive scattered light generated from the reaction solution at different light-receiving angles, a first data processing unit configured to process reaction process data measured by one of the light receivers to quantitatively determine a concentration of a substance in the reaction solution, and a second data processing unit configured to determine if the quantitative determination of the concentration of the substance has been performed normally on the basis of a ratio of a plurality of computed values, the plurality of computed values having been calculated from a plurality of pieces of reaction process data measured by the respective light receivers.