Schizochytrium DHA Production via Solid Culture and Liquid Fermentation
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Solution Overview
Problem
Traditional methods for producing DHA through Schizochytrium fermentation are complex, time-consuming, and costly, with multiple fermentation stages and high investment costs, necessitating a simplification of the seed preparation process and reduction in production costs.
Innovation Solution
A method involving solid culture and liquid fermentation of Schizochytrium, which simplifies the seed enlargement process, shortens the culture and fermentation period, and reduces investment by using a eutrophic solid culture medium rich in growth factors, resulting in strong cell viability and reduced waste and environmental pollution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional multi-stage fermentation method is used, then DHA production is achieved, but the process is complex and time-consuming
Solution Approach 1:
The patent combines multiple fermentation stages (activation, seed culture, and fermentation) into a streamlined process by using solid culture medium directly in shake flasks for seed preparation, eliminating the need for separate activation and seed tank stages. This merging of operations reduces process complexity while maintaining DHA production efficiency.
Solution Approach 2:
The patent performs preliminary action by preparing optimized solid culture medium containing all necessary nutrients and growth factors before inoculation. The solid culture medium is pre-formulated with appropriate carbon sources, nitrogen sources, and trace elements to support direct fermentation, eliminating the need for sequential medium preparations in traditional methods.
2Productivity
If traditional multi-stage fermentation method is used, then DHA production is achieved, but the culture period is long
Solution Approach 1:
The patent skips the traditional intermediate seed culture stage by directly inoculating the strain into solid culture medium in shake flasks. This rushing through of the seed preparation phase reduces the overall culture period while still achieving adequate biomass and DHA production for fermentation scale-up.
Solution Approach 2:
The patent changes the physical state of the culture medium from liquid to solid form, which alters the growth characteristics and reduces the culture period. The solid culture medium provides better oxygen transfer and prevents contamination, enabling faster growth rates and shorter culture times while maintaining high DHA production.
3Productivity
If traditional fermentation method is used, then DHA production is achieved, but investment cost is high
Solution Approach 1:
The patent uses disposable solid culture medium in shake flasks for seed preparation, eliminating the need for expensive sterilization equipment and multiple fermentation tanks. The solid culture medium can be easily prepared and disposed of, reducing capital investment and operational costs while maintaining effective seed production for fermentation.
Solution Approach 2:
The patent extracts and eliminates the complex multi-stage fermentation infrastructure (activation tanks, seed tanks, fermentation tanks) by performing all seed preparation and initial fermentation in simple shake flasks with solid culture medium. This extraction of unnecessary equipment reduces investment costs while preserving the essential DHA production function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the yield of DHA, reduces production costs, and is more suitable for large-scale production, while minimizing waste and environmental impact, providing a stable and efficient method for producing DHA as a nutritional supplement.
Implementation Method 1
a method for producing DHA through solid culture and liquid fermentation of Schizochytrium
Data Source
AI summary
The invention relates to a method for fermentation production of DHA, in particular a method for producing DHA through solid culture and liquid fermentation of Schizochytrium. The method comprises steps of activation of a strain, preparation of a suspension of the strain, preparation of a primary seed, preparation of a secondary solid seed, enlarging cultivation of the secondary solid seed in a fermentor, collection of cells after fermentation, and extraction of DHA oil. With the fermentation method, the seed preparation method is improved, the seed enlarging technology is simplified, the period of seed culture and fermentation is shortened, and therefore the investment is reduced and the cost is saved; in addition, the solid culture medium is of eutrophy and rich in a plurality of growth factors, and is more beneficial to the growth of the seed; cells are strong in viability and good in synchronism in solid culture medium.