Single-Domain Antibody Binding G Protein Alpha
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current tools are inadequate for specifically detecting the alpha subunit of the G protein, particularly for use in FRET processes, and there are no effective single-domain antibodies (sdAbs) that bind to the G protein alpha.
Innovation Solution
Development of single-domain antibodies (sdAbs) with specific amino acid sequences that bind to the G protein alpha, featuring a dissociation constant of less than 100 nM, allowing for effective detection in FRET processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional detection tools are used, then detection capability is provided, but specificity for G protein alpha subunit is insufficient
Solution Approach 1:
The invention uses single-domain antibodies (sdAbs) that specifically target and bind to the alpha subunit of G proteins, segmenting the detection function to achieve high specificity for this particular protein subunit while maintaining overall detection reliability
Solution Approach 2:
The sdAbs act as intermediary molecules that specifically recognize and bind to the G protein alpha subunit, enabling precise detection in FRET processes by mediating between the target protein and the detection system
2Measurement precision
If single-domain antibodies are developed with high affinity binding, then detection precision is improved, but development complexity increases
Solution Approach 1:
The invention optimizes the dissociation constant parameter of the sdAbs to achieve high affinity binding (Kd < 100 nM), thereby improving detection precision while managing development complexity through focused parameter optimization
Data Source
AI summary
The present invention relates to a single-domain antibody (sdAb) which binds to the G protein alpha, comprising an amino acid sequence consisting of 3 CDR regions (CDR1 to CDR3) and 4 hinge regions (FR1 to FR4) according to the following formula (I): FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 (I) and advantageously having a dissociation constant (Kd), measured in FRET, of less than 100 nM.