SDC2 Methylation Detection With Specific Primers and Probes
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Solution Overview
Problem
Existing methods for detecting methylation of the SDC2 gene are not sufficiently sensitive and specific for early diagnosis of colorectal cancer, particularly in samples like stool and blood, limiting their effectiveness in cancer diagnosis and prognosis.
Innovation Solution
A method using a primer specifically amplifying methylated SDC2 gene and a probe capable of complementary hybridization to the methylated SDC2 gene, combined with reagents that modify cytosine bases, allowing for accurate detection of methylation in samples such as colorectal tissue, stool, and blood.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If existing methods for detecting methylation of SDC2 gene are used, then detection can be performed, but sensitivity and specificity are insufficient for early diagnosis of colorectal cancer
Solution Approach 1:
The patent applies parameter changes by modifying the detection methodology parameters - using methylation-specific PCR with optimized primers and probes that target specific CpG sites in the SDC2 gene promoter region. This changes the detection parameters from generic methylation detection to highly specific detection of methylated SDC2 gene sequences, thereby improving both sensitivity and specificity for early colorectal cancer diagnosis
Solution Approach 2:
The patent replaces conventional detection methods with a molecular biology-based detection system using PCR amplification and hybridization probes. This substitution of detection mechanism enables highly sensitive and specific detection of methylated DNA sequences in stool and blood samples, overcoming the limitations of existing methods
2Ease of operation
If methylation detection is performed in stool and blood samples, then non-invasive diagnosis is enabled, but detection sensitivity is limited
Solution Approach 1:
The patent uses stool and blood samples as intermediary materials that contain cell-free DNA or shed cells from the gastrointestinal tract. The methylation-specific PCR detection method acts as an intermediary detection system that can identify methylated SDC2 gene sequences in these intermediary samples, enabling non-invasive diagnosis while maintaining high detection sensitivity through specific molecular detection
Solution Approach 2:
The patent changes the detection parameters to optimize for low-concentration targets in complex matrices like stool and blood. By using highly specific primers and probes targeting methylated CpG sites in the SDC2 gene promoter, the method achieves sufficient sensitivity despite the challenging sample types, maintaining both ease of operation and measurement precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high sensitivity and specificity for detecting SDC2 gene methylation, enabling effective early diagnosis of colorectal cancer with improved diagnostic accuracy in various sample types.
Implementation Method 1
a primer specifically amplifying a methylated SDC2 gene
Implementation Method 2
a probe capable of complementary hybridization to the methylated SDC2 gene specifically amplified by the primer
Implementation Method 3
treating a sample with at least one reagent differently modifying a methylated SDC2 gene and a non-methylated SDC2 gene
Data Source
AI summary
The present invention relates to a method of detecting methylation of an SDC2 gene, a composition for detecting methylation of an SDC2 gene, and a kit comprising same and, more particularly, to: a method of detecting methylation of an SDC2 gene by using primers for specifically amplifying a methylated SDC2 gene and a probe capable of complementarily hybridizing with a methylated SDC2 gene that has been specifically amplified by the primers; a composition for detecting methylation of an SDC2 gene; and a kit comprising same.