sdLDL Quantification Reagent Composition for Selective LDL Reaction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for measuring small dense LDL cholesterol (sdLDL-C) lack accuracy.
Innovation Solution
A reagent composition comprising polyoxyethylene monostyrenated phenyl ether with specific enzyme activities and surfactant properties is used to selectively act on LDLs other than sdLDL, followed by a second reagent composition for quantifying sdLDL-C, enhancing measurement accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional reagents are used for HPV detection, then the detection process requires multiple separate steps (nucleic acid extraction, amplification, hybridization), but this increases the time required for detection and reduces workflow efficiency
Solution Approach 1:
The patent combines multiple separate detection steps (nucleic acid extraction, amplification, and hybridization) into a single multiplexed reaction system. The reagent composition enables simultaneous amplification of multiple HPV gene targets and hybridization with detection probes in one reaction mixture, eliminating the need for sequential processing and significantly reducing detection time while maintaining accuracy.
Solution Approach 2:
The reagent composition is designed to perform multiple functions simultaneously: it serves as both the amplification reagent and the hybridization probe system. The composition can detect multiple HPV genotypes (including high-risk and low-risk types) in a single reaction, providing universal detection capability across different HPV strains without requiring separate reagent systems for each target.
2Adaptability or versatility
If multiple separate reagents are used for different detection targets, then detection coverage is comprehensive, but reagent storage and management become complex and prone to errors
Solution Approach 1:
The patent merges multiple detection targets into a single reagent composition. The multiplexed system includes primers and probes for detecting multiple HPV genotypes (such as HPV 16, 18, 31, 33, 45, 52, 58 and other types) all contained in one reagent kit, eliminating the need to manage multiple separate reagents for different targets and reducing storage and handling complexity.
Solution Approach 2:
The reagent composition provides universal detection capability for a broad spectrum of HPV genotypes through a single multi-functional system. The design allows simultaneous detection of high-risk and low-risk HPV types using the same reagent mixture, enhancing versatility while simplifying reagent inventory management and reducing the risk of selection errors.
3Measurement precision
If conventional amplification and detection methods are used separately, then each step can be optimized independently, but the overall detection cost increases and sample consumption increases
Solution Approach 1:
The patent combines the amplification and detection steps into a single multiplexed reaction, allowing the same sample to be used for both purposes simultaneously. This eliminates the need to divide samples into separate aliquots for amplification and hybridization, reducing total sample consumption while maintaining the precision of both detection methods through optimized reagent concentrations and reaction conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for accurate quantification of sdLDL-C by stabilizing the action of the first reagent composition on LDLs other than sdLDL, thereby improving measurement precision.
Implementation Method 1
the probe is specifically hybridized to a target sequence
Implementation Method 2
the probe is specifically hybridized to a target sequence, and the streptavidin is specifically bound to the probe
Data Source
Figure 1(a)~1(c)
Figure 2(d)~2(f)
Figure 3(g)~3(i)
AI summary
A reagent composition that is used as a first reagent composition for a method of quantifying small dense LDL cholesterol (sdLDL-C) in a sample, the method including causing the first reagent composition to act on the sample, and after the causing the first reagent composition to act on the sample, applying a second reagent composition for quantifying the sdLDL-C to quantify cholesterol in a remaining lipoprotein, in which the reagent composition has one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity, and contains polyoxyethylene monostyrenated phenyl ether.