Sealed Microfluidic Bio Detection With Centrifugal Flow Control
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Solution Overview
Problem
Current biological detection devices suffer from low integration, limited versatility, poor feasibility, compromised detection performance, and high risks of cross-contamination, necessitating manual operations and complex sample handling.
Innovation Solution
A whole-process biological detection device integrating sample-loading, lysis, extraction, amplification, and detection units with controlled fluid flow and reagent storage, utilizing centrifugal force for automated operation and sealed containment to prevent cross-contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If manual operations are used for sample handling and reagent addition, then flexibility in handling different samples is maintained, but the risk of cross-contamination increases and operation complexity increases
Solution Approach 1:
The microfluidic chip is designed with integrated reagent storage chambers and automated fluid control channels that enable the system to perform sample handling, reagent addition, and workflow control autonomously without manual intervention, thereby eliminating cross-contamination risks while maintaining versatility through programmable fluid routing
Solution Approach 2:
The chip integrates multiple functional modules including sample loading, reagent storage, lysis, extraction, amplification, and detection within a single device, allowing it to handle various sample types and perform complete nucleic acid detection workflows automatically, achieving both versatility and contamination prevention
2Adaptability or versatility
If nucleic acid extraction and purification modules are added to handle complex samples, then detection sensitivity and versatility improve, but device complexity and manufacturing difficulty increase
Solution Approach 1:
The lysis, extraction, and purification functions are merged into a single integrated module within the microfluidic chip, where magnetic bead-based extraction is combined with automated washing and elution channels, achieving complex sample处理能力 without proportionally increasing device complexity through modular integration
Solution Approach 2:
Multiple functional chambers including sample loading, reagent storage, lysis, magnetic bead extraction, washing, and elution are nested within the compact microfluidic chip structure, allowing complex functionality to be contained in a small integrated device with hierarchical organization of sub-functions
3Extent of automation
If integrated whole-process modules are implemented, then automation level and contamination prevention improve, but device complexity and operational reliability requirements increase
Solution Approach 1:
The automated detection system is segmented into distinct functional modules (sample loading, lysis, extraction, amplification, detection) connected by controlled fluid channels, allowing each module to be independently optimized and tested, which simplifies reliability verification while maintaining high automation and contamination prevention
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves high integration, automated operation, and reduced cross-contamination risks, ensuring efficient and reliable biological detection with simplified sample handling and reduced biosafety hazards.
Implementation Method 1
a microfluidic chip device for nucleic acid detection based on centrifugal drive
Data Source
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AI summary
A whole-process biological detection device is provided. The whole-process biological detection device belongs to the technical field of biological detection, and includes at least one reactor (100), where the reactor includes at least one sample-loading unit (1), at least one liquid release-control unit (2), at least one extraction unit (3), at least one liquid switch-control unit (4), at least one liquid collection unit (5), at least one liquid transfer unit (6), at least one liquid flow-control unit (7), at least one liquid quantitative dispersion unit (8) and multiple reaction cells (9). The product of the solution has the characteristics of high integration, no need for additional reagents, simple operation, stable and reliable, etc., thereby achieving the goal of sealing the whole process of biological detection and effectively avoiding cross-contamination between samples and equipment.