Sealed Operation Pipe for Nucleic Acid Extraction
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Solution Overview
Problem
Existing methods for nucleic acid extraction and purification are prone to contamination due to aerosol generation during liquid collection, require complex and expensive devices, and often necessitate separate systems for analysis.
Innovation Solution
A sealable operation pipe filled with liquid reagents partitioned by a water-insoluble gel, where magnetic particles are moved by an external magnetic field to perform extraction, purification, and analysis without aerosol generation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If liquid collection is performed by pipetting operations, then liquid transfer is achieved, but aerosols are generated causing contamination
Solution Approach 1:
The patent replaces mechanical pipetting operations with magnetic field-based manipulation of magnetic particles. Magnetic particles are used to collect and transfer liquids through magnetic actuation, eliminating the need for physical pipette contact with samples and thus preventing aerosol generation and contamination.
Solution Approach 2:
The patent introduces magnetic particles as an intermediary substance between the operator and the liquid sample. These magnetic particles serve as a mediator that can be manipulated by external magnetic fields to perform liquid collection and transfer operations without direct mechanical contact, thereby avoiding contamination.
2Adaptability or versatility
If separate systems are used for extraction and analysis, then specialized functions are achieved, but device complexity increases
Solution Approach 1:
The patent combines extraction and analysis functions into a single integrated device. The same magnetic particle-based platform is used for both liquid collection/extraction and subsequent analysis operations, eliminating the need for separate systems and reducing overall device complexity while maintaining specialized functionality.
Solution Approach 2:
The patent creates a universal magnetic particle-based system that can perform multiple functions including liquid collection, extraction, purification, and analysis. This multi-functional approach allows a single device to replace multiple specialized systems, reducing complexity while maintaining adaptability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate recovery rate evaluation of nucleic acids with reduced contamination risk, simplified device design, and integrated analysis capabilities within a sealed system.
Implementation Method 1
magnetic particles are moved by an external magnetic field to perform extraction, purification, and analysis
Implementation Method 2
A sealable operation pipe filled with liquid reagents partitioned by a water-insoluble gel
Data Source
AI summary
A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.