Secreted eIF5A Protein for Oxidative Stress Diagnosis and Apoptosis Control

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Solution Overview

Problem

Current methods fail to effectively diagnose and treat diseases induced by oxidative stress, particularly in conditions like ischemia/reperfusion injury, where apoptosis is triggered by mechanisms independent of neutrophil infiltration, and existing proteins like eIF5A are not known to be secreted extracellularly.

Innovation Solution

Identification of secreted eIF5A as a novel protein that acts as an apoptosis-inducing ligand under oxidative stress conditions, allowing for its measurement in fluids or tissues to diagnose oxidative stress and develop therapeutic agents to inhibit apoptosis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If eIF5A is used as a translation initiation factor in the cytoplasm, then cell proliferation is promoted, but it cannot be used for diagnosing oxidative stress or inducing apoptosis

Engineering Contradiction:

Improvediagnostic accuracy for oxidative stressVSAvoidfunctional role of eIF5A

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The invention segments eIF5A into two distinct forms: cytoplasmic eIF5A (for translation initiation and cell proliferation) and secreted eIF5A (for oxidative stress response and apoptosis induction). This segmentation allows each form to perform its specific function without interference, resolving the contradiction between maintaining eIF5A's original cellular function and enabling its use as a diagnostic marker.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

Secreted eIF5A acts as an intermediary molecule that mediates the response to oxidative stress. It is released from cells under oxidative stress conditions and induces apoptosis in target cells, serving as a signaling molecule that bridges the gap between oxidative stress detection and apoptotic response.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If secreted eIF5A is used to induce apoptosis under oxidative stress, then therapeutic benefit is achieved, but the mechanism is not yet understood

Engineering Contradiction:

Improvetherapeutic effectivenessVSAvoidmechanism understanding

Core Design Contradiction:

ReliabilityVSLoss of information

Solution Approach 1:

The invention establishes a feedback mechanism where secreted eIF5A levels provide information about oxidative stress status. By measuring secreted eIF5A in body fluids, the system feedbacks the degree of oxidative stress, enabling both diagnosis and monitoring of therapeutic response while gradually elucidating the mechanism through observable correlations.

Inventive Principle:

Principle #23Feedback

3Adaptability or versatility

If conventional methods are used to diagnose oxidative stress, then existing diagnostic limitations persist, but new diagnostic markers are not identified

Engineering Contradiction:

Improvediagnostic capabilityVSAvoiddiagnostic accuracy

Core Design Contradiction:

Adaptability or versatilityVSReliability

Solution Approach 1:

The invention changes the diagnostic parameter from conventional oxidative stress markers to secreted eIF5A protein levels. This parameter change enables more accurate and specific diagnosis of oxidative stress conditions, as secreted eIF5A directly reflects the cellular response to oxidative stress and its apoptotic effects.

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS9321819B2Oxidative stress responsive apoptosis inducing protein eIF5A

Publication Date: 2016.04.26 SEKO YOSHINORI

AI summary

Secreted eIF5A protein which is useful for diagnosis, prevention, and treatment of various diseases induced by an oxidative stress.