Seed-Bred Cannabis Varieties With High CBD and Low THC Stability
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Solution Overview
Problem
Existing Cannabis breeding methods struggle to produce varieties that consistently express low THC levels and elevated levels of certain cannabinoids, particularly CBD, while maintaining stability and high yield under diverse environmental conditions.
Innovation Solution
Development of Cannabis varieties NWG 4000 and NWG 2463 using recurrent selection and mass selection breeding methods, combined with genetic engineering techniques to introduce desired traits such as low THC and high CBD content, achieved through crossing and backcrossing with NWG 988 and NWG 452, and utilizing molecular markers for trait selection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If clonal propagation is used to produce Cannabis varieties, then breeding simplicity is improved, but production cost increases
Solution Approach 1:
The patent changes the fundamental parameter of reproduction method from clonal propagation to seed-based reproduction. This allows the variety to be propagated through seeds while maintaining consistent cannabinoid profiles, thereby reducing production costs associated with clonal methods while preserving breeding simplicity through selective breeding programs.
Solution Approach 2:
The patent creates a stable, reproducible genetic copy of the desired cannabinoid profile through selective breeding. By establishing a consistent genetic lineage that reliably produces low-THC, high-CBD Cannabis plants, the variety can be replicated through seed production rather than requiring expensive clonal propagation methods.
2Reliability
If Cannabis varieties are bred for elevated THC levels, then medicinal properties are improved, but regulatory compliance deteriorates
Solution Approach 1:
The patent extracts and isolates the desired cannabinoid CBD from the Cannabis plant while deliberately excluding or minimizing THC content. Through selective breeding, the variety is developed to produce high levels of medicinal CBD while keeping THC below regulatory thresholds, effectively separating the beneficial properties from the harmful or regulated components.
Solution Approach 2:
The patent applies local quality by creating a Cannabis variety with non-uniform cannabinoid distribution characteristics. The plant produces elevated levels of specific cannabinoids (CBD) in certain tissues while maintaining low levels of others (THC), allowing different parts of the plant to have different cannabinoid profiles suited for medicinal applications without violating regulations.
3Quantity of substance
If Cannabis varieties are bred for high CBD content, then medicinal value is improved, but THC levels may increase beyond acceptable thresholds
Solution Approach 1:
The patent simultaneously changes multiple cannabinoid parameters through selective breeding. The variety is developed to achieve high CBD content while maintaining low THC levels, effectively optimizing the ratio and absolute levels of multiple cannabinoids at once rather than treating them as separate traits.
4Reliability
If Cannabis varieties are bred for consistent cannabinoid expression, then reliability is improved, but genetic stability becomes more difficult to maintain
Solution Approach 1:
The patent creates a stable genetic copy or lineage that reliably transmits the desired cannabinoid profile across generations. Through repeated selective breeding and population management, a consistent genetic foundation is established that ensures uniform cannabinoid expression while maintaining genetic stability within the variety.
Data Source
AI summary
Novel Cannabis varieties designated NWG 4000 and NWG 2463 are provided. This disclosure thus relates to the seeds of Cannabis variety NWG 4000 or NWG 2463, to the plants of Cannabis variety NWG 4000 or NWG 2463, to plant parts of Cannabis variety NWG 4000 or NWG 2463, to methods for producing a Cannabis plant by crossing a plant of Cannabis variety NWG 4000 or NWG 24633 with a plant of another Cannabis variety, and to methods for producing a plant of Cannabis variety NWG 4000 or NWG 2463 containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion Cannabis plants and plant parts produced by those methods.