Segmented Quantum Dot Test Cards for Multiplex Allergen Quantification
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Solution Overview
Problem
Current commercially available products for quantitative immunochromatographic testing of allergens face challenges in performing simultaneous multiplex testing and suffer from severe interference during multiplex testing.
Innovation Solution
A multiplex quantum dot-based quantitative test card and apparatus that includes a multiplex card housing with reaction slots, sample loading ports, and observation windows, featuring a test strip with a conjugate pad coated with quantum dot-labeled antibodies, and a nitrocellulose membrane with uniformly spaced test lines and a control line, enabling simultaneous multiplex testing with reduced interference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If quantum dot-based quantitative immunochromatographic testing is used, then measurement precision and reliability are improved, but device complexity increases due to the need for multiplex testing capabilities
Solution Approach 1:
The test card is divided into multiple reaction slots, each capable of independently accommodating a test strip for different allergens. This segmentation allows simultaneous multiplex testing of multiple allergens in parallel, improving measurement precision while managing device complexity through modular design
Solution Approach 2:
The multiplex card housing is designed with universal functionality to accommodate multiple test strips with different allergen-specific quantum dot-labeled antibodies. The single sample loading port can service multiple reaction slots, enabling one sample to be tested for multiple allergens simultaneously, thereby improving measurement precision without proportionally increasing operational complexity
2Productivity
If multiple allergens are tested simultaneously in a single sample, then productivity is improved, but object-affected harmful factors increase due to interference between test items
Solution Approach 1:
The test card physically segments multiple test reactions into separate reaction slots, each with its own test strip. This spatial segmentation prevents interference between different allergen testing while allowing simultaneous processing, thereby improving productivity without introducing harmful interactions between test items
Solution Approach 2:
The nitrocellulose membrane acts as an intermediary medium that selectively binds quantum dot-labeled antibodies specific to each allergen. This intermediary mechanism ensures that each test item reacts only with its corresponding allergen, preventing cross-reactivity and interference while enabling simultaneous multiplex testing
3Measurement precision
If quantum dot-labeled antibodies are used for chromogenic substances, then measurement precision is improved through enhanced fluorescence signal, but manufacturing precision becomes more difficult due to the complexity of quantum dot labeling
Solution Approach 1:
The quantum dot-labeled antibodies are prepared in advance during the manufacturing phase, with the labeling process completed before the test card is assembled. This preliminary action allows for controlled and consistent labeling conditions, improving measurement precision while managing manufacturing complexity by concentrating the difficult labeling step during production rather than during use
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, accurate, and efficient quantitative testing of allergen-specific IgE antibodies, overcoming multiplex testing challenges and interference, suitable for diagnosing allergic diseases with high sensitivity and user-friendly operation.
Implementation Method 1
Quantum dots (QDs), as a novel class of fluorescent nanocrystals, exhibit high fluorescence efficiency, photobleaching resistance, long fluorescence lifetime, narrow and symmetrical emission spectra, single-wavelength excitation with multiplexed emissions
Implementation Method 2
fluorescent immunoassay technology, which utilizes fluorescently labeled antibodies or antigen molecules to quantitatively analyze test samples by detecting changes in fluorescence signal intensity following specific binding
Implementation Method 3
the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances
Data Source
AI summary
Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.