Selective Deletion Chromatography for Complex Mixture Modulation
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Solution Overview
Problem
There is a lack of accessible methodologies for deconvoluting complex mixtures from botanical and natural extracts without laborious purification of individual components, and for understanding the holistic interactions of their constituents, particularly in assessing therapeutic efficacy and modifying these mixtures for specific therapeutic effects.
Innovation Solution
The use of selective deletion chromatography methods, such as fractionating extracts by liquid chromatography, where a minor fraction is diverted for detection and a majority fraction is collected, allowing for the removal or addition of components to create refined or tuned compositions with specific therapeutic effects, applied to plant sources like green tea extracts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If laborious purification of individual components is used to deconvolute complex mixtures, then measurement precision and understanding of individual constituents is improved, but device complexity and loss of time increase significantly
Solution Approach 1:
The patent segments the complex mixture analysis into two parallel streams: (1) a minor fraction diverted to detectors for component identification and (2) a majority fraction collected for subsequent analysis. This segmentation allows simultaneous component identification and mixture collection without sequential purification steps, dramatically reducing analysis time while maintaining precision.
Solution Approach 2:
The patent introduces an intermediary detection system (detectors, mass spectrometry) that analyzes a small diverted fraction of the eluant to identify components. This intermediary provides the information needed to understand the complex mixture without requiring complete purification of all components, thus saving time while maintaining measurement precision.
2Measurement precision
If complete purification of all components is performed to understand holistic interactions, then measurement precision is improved, but device complexity and loss of substance increase
Solution Approach 1:
The patent extracts only the necessary information about mixture components and their interactions by analyzing a small diverted fraction (minor eluant stream) while collecting the majority fraction for subsequent holistic analysis. This selective extraction provides sufficient data to understand component interactions without requiring complete purification and loss of the entire mixture.
Solution Approach 2:
The patent performs partial purification by diverting and analyzing only a minor fraction (e.g., 5-10%) of the eluant stream. This partial action provides sufficient information about component interactions and identities without the excessive action of completely purifying and analyzing every component, thereby minimizing substance loss while maintaining analytical precision.
3Adaptability or versatility
If selective deletion chromatography is used to modify complex mixtures, then adaptability for specific therapeutic effects is improved, but device complexity increases
Solution Approach 1:
The patent employs dynamic control of the chromatography system to selectively delete or retain specific components based on real-time detector feedback. The system can dynamically adjust which components are removed or concentrated in the collected fractions, providing adaptability for different therapeutic applications without requiring multiple fixed systems.
Solution Approach 2:
The patent utilizes parameter changes in the chromatography process (mobile phase composition, flow rate, temperature) to selectively modulate which components are deleted or retained. By changing these parameters, the system can be adapted for different therapeutic effects using the same basic apparatus, managing complexity while maximizing versatility.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the modulation of complex mixtures for enhanced or decreased bioactive properties, allowing for the characterization and optimization of natural extracts, providing compositions that can treat conditions associated with reactive oxygen species and inflammatory molecules like TNFα.
Implementation Method 1
fractionating an extract sample by liquid chromatography; applying a liquid chromatography system comprising a chromatography column; applying a mobile phase to the chromatography column
Data Source
AI summary
The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNFα, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.