Semi-solid Aqueous Enzymatic Rapeseed Oil Extraction
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Solution Overview
Problem
Traditional aqueous enzymatic methods for producing rapeseed oil suffer from low yield, emulsification issues, and loss of trace nutrients due to high water content, which complicates the separation process and reduces the retention of active ingredients like tocopherols, sterols, and beta-carotene.
Innovation Solution
A semi-solid aqueous enzymatic process involving rapeseeds with adjusted moisture content, enzyme treatment at controlled temperatures, and specific enzyme ratios (pectinase:cellulase:alkaline protease) to enhance oil yield and retention of nutrients, using a 1:1 oil-producing powder to water ratio and centrifugation to separate crude oil effectively.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional aqueous enzymatic method with high water content is used, then the hydrolysis reaction can proceed smoothly, but the yield of clear oil is low and emulsification problems occur
Solution Approach 1:
The patent changes the water content parameter from traditional high water content (ratio 1:4-6) to controlled water content (ratio 1:1 to 1:2), transforming the reaction medium from liquid to semi-solid state. This parameter change resolves the contradiction by enabling smooth hydrolysis while preventing emulsification and improving clear oil yield up to 93.5%
Solution Approach 2:
The patent dynamically adjusts the water content during the process, initially using sufficient water for enzymatic hydrolysis, then removing excess water through heating and centrifugation. This dynamic control of water content allows the system to transition from a water-rich state favorable for hydrolysis to a water-controlled state that prevents emulsification
2Quantity of substance
If high water content is used in traditional aqueous enzymatic method, then the enzymatic hydrolysis can be carried out, but trace nutrients like tocopherols, sterols, and beta-carotene are lost due to dissolution in water
Solution Approach 1:
The patent changes the water content parameter to a controlled semi-solid state (ratio 1:1 to 1:2), which is sufficient for enzymatic hydrolysis but insufficient to dissolve trace nutrients. This resolves the contradiction by maintaining process feasibility while preventing nutrient loss through dissolution, achieving retention of tocopherols, sterols, and beta-carotene
Solution Approach 2:
The patent uses a semi-solid matrix that copies the essential function of water for enzymatic activity while eliminating the harmful effect of water dissolution on trace nutrients. The controlled water content provides the necessary medium for enzymes while preventing nutrient leaching
3Productivity
If traditional liquid state hydrolysis is used, then the reaction proceeds easily, but further demulsification treatment is required which consumes energy and time
Solution Approach 1:
The patent performs preliminary water removal through heating and centrifugation during the hydrolysis process itself, before the separation stage. This preliminary action prevents emulsion formation in the first place, eliminating the need for subsequent energy-intensive demulsification treatments and improving overall process efficiency
Solution Approach 2:
The patent converts the potential harm of water presence (emulsification and nutrient loss) into a benefit by using controlled water content to facilitate enzymatic hydrolysis while preventing emulsion formation. The semi-solid state allows easy separation without additional demulsification steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a high clear oil yield of up to 93.5% with significantly higher retention of tocopherols, sterols, and beta-carotene, and demonstrates health benefits in reducing body weight, fat accumulation, and serum triglycerides and cholesterol levels in hyperlipidemic rats.
Implementation Method 1
Aqueous enzymatic hydrolysis method uses proteases to hydrolyze oil bodies and separates proteins and oils by centrifugation
Implementation Method 2
0.5% to 2% (w/w) of the total enzyme (pectinase:cellulase:alkaline protease=1:1:1, by weight) is added to the semi-solid rapeseed paste
Implementation Method 3
The rapeseed paste is then centrifuged to separate crude oil from solid residues
Implementation Method 4
stirring and mixing at 40-60° C. for 1-2 hr
Data Source
AI summary
The invention discloses a method for preparing rapeseed oil by a semi-solid aqueous enzymatic process, belonging to the field of functional foods and health care products. The present invention first prepares a semi-solid rapeseed paste with 3.5-4.5% moisture content, which is hydrolyzed by a mixture of pectinase, cellulase and alkaline protease to extract rapeseed oil. The resulting rapeseed oil contains high levels of active ingredients including totaxin, sterol, phenols and beta-carotene. The rapeseed oil of the invention can be added into animal feeds, which helps to reduce animal blood lipid levels and body weight. It can significantly reduce the contents of total triglyceride, total cholesterol and LDC-C in the blood, and, at the same time, increases the level of HDL-C in the blood. In addition, the rapeseed oil prepared by the present invention can relieve hepatic steatosis in hyperlipemia rats.