Seminal Plasma Protein Detection via Capture Reagent Chips
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Solution Overview
Problem
Current methods for detecting male fertility-associated proteins are limited, failing to systematically analyze protein expression levels in seminal plasma, which is crucial for understanding male infertility, as they primarily focus on genetic levels and do not account for post-transcriptional regulation or protein modifications.
Innovation Solution
Development of capture reagents, specifically antibodies, for detecting male fertility-associated proteins using protein chips, which allow for qualitative and quantitative analysis of seminal plasma and sperm samples, utilizing markers like fluorescence, enzyme markers, and biotin to identify key proteins involved in sperm maturation and function.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of information
If traditional genetic-level detection methods are used, then the detection process is simple, but the information regarding post-transcriptional regulation and protein expression levels cannot be obtained
Solution Approach 1:
The invention segments the detection process into multiple specialized components: capture reagents for immobilizing target proteins, detection reagents with specific markers (fluorescence, enzyme, biotin, isotopes) for signal generation, and a detection device for reading results. This segmentation allows comprehensive protein detection while maintaining operational simplicity through modular design.
Solution Approach 2:
The invention introduces capture reagents as intermediaries that specifically bind to target proteins in seminal plasma, forming a stable complex that enables subsequent detection. These capture reagents act as mediators between the complex biological sample and the detection system, facilitating accurate protein level measurement while accounting for post-transcriptional regulation.
2Measurement precision
If routine semen detection methods are used, then the detection is quick and simple, but the expression level for proteins in seminal plasma cannot be analyzed systemically
Solution Approach 1:
The invention merges multiple detection functions into a single integrated detection system that can simultaneously analyze multiple protein targets in seminal plasma. By combining capture reagents, detection reagents with various markers, and a unified detection device, the system achieves comprehensive protein expression analysis while reducing total detection time compared to sequential individual protein assays.
Solution Approach 2:
The detection device is designed with universal functionality to detect multiple types of markers (fluorescence, enzyme, biotin, isotopes) through a single platform. This multi-functionality allows systematic analysis of various protein expression levels in seminal plasma without requiring separate specialized equipment for each protein or marker type, thereby improving measurement precision while maintaining efficiency.
3Measurement precision
If mRNA level detection is used, then the detection method is established, but the protein expression abundance cannot be accurately reflected
Solution Approach 1:
The invention replaces indirect mRNA-level detection with direct protein-level detection using physical-chemical methods. Detection reagents with markers (fluorescence, enzyme, biotin, isotopes) provide direct measurement of protein abundance through optical, chemical, or radioactive signals, eliminating the need to infer protein levels from mRNA data and accurately reflecting post-transcriptional regulation effects.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables comprehensive detection of multiple male fertility-associated proteins, aiding in the identification of causes of male infertility by providing insights into protein expression and function, thereby improving diagnostic efficiency and understanding of sperm maturation mechanisms.
Implementation Method 1
the use of a group of capture reagents for human sperm maturation and male fertility-associated proteins... wherein said capture reagents are used to prepare chips or kits for detecting expression of male fertility-associated proteins
Implementation Method 2
said markers generally include immunofluorescence marker, enzyme marker, silver stain, biotin, isotopes, etc.
Implementation Method 3
said markers generally include immunofluorescence marker, enzyme marker, silver stain, biotin, isotopes, etc.
Implementation Method 4
said markers generally include immunofluorescence marker, enzyme marker, silver stain, biotin, isotopes, etc.
Data Source
AI summary
A detection method for 305 fertility-associated sperm localization protein expressed in human testis and epididymis. The method includes qualitatively or quantitatively detecting for sperm localization proteins on a sperm or in seminal plasma via capture agents for the sperm localization proteins. The sperm localization proteins belong to an important protein group related to human sperm maturation and fertility. The method provides a diagnostic approach for male infertility.