Sense Oligonucleotide iNOS mRNA Suppression
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Solution Overview
Problem
Current methods lack effective control over the expression of inducible nitric oxide synthase (iNOS) to manage excessive production of nitric oxide, which contributes to inflammatory diseases and other disorders, as the existing RNA interference techniques are not specifically tailored for iNOS regulation.
Innovation Solution
Development of sense oligonucleotides with sequences complementary to iNOS mRNA antisense transcripts, which stabilize and interfere with the mRNA, thereby suppressing the expression of iNOS and reducing excessive NO production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If RNA interference techniques are used to control iNOS expression, then gene expression can be suppressed, but the methods lack specificity and effectiveness for iNOS regulation
Solution Approach 1:
The patent applies local quality by designing oligonucleotides with specific sequences that target only the 3' UTR region of iNOS mRNA. This region-specific targeting ensures that the suppression effect is localized to iNOS expression without affecting other genes, thereby achieving both reliability in iNOS control and specificity for iNOS regulation.
Solution Approach 2:
The patent uses antisense RNA and sense oligonucleotides as intermediary molecules that bind to iNOS mRNA in the 3' UTR region. These intermediaries mediate the suppression of iNOS expression by forming RNA-RNA hybrids that prevent mRNA translation or promote degradation, providing a specific and effective mechanism for iNOS regulation.
2Quantity of substance
If excessive NO production is allowed to occur, then inflammatory response can be mounted, but toxic effects and tissue damage result
Solution Approach 1:
The patent implements a feedback mechanism by using oligonucleotides that specifically bind to iNOS mRNA and suppress its expression. This creates a negative feedback loop where excessive iNOS expression is detected and automatically suppressed through the oligonucleotide-mRNA interaction, preventing toxic NO accumulation while allowing appropriate inflammatory response.
Solution Approach 2:
The patent converts the harmful effect of excessive NO production into a beneficial therapeutic outcome by using the same iNOS mRNA that causes the problem as the target for suppression. The oligonucleotides exploit the presence of iNOS mRNA (which indicates excessive expression) to deliver targeted suppression, turning the harmful situation into a controlled therapeutic intervention.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of these sense oligonucleotides effectively decreases iNOS mRNA levels, reducing excessive NO production and addressing inflammatory diseases and disorders related to iNOS overexpression.
Implementation Method 1
sense oligonucleotide having a sequence complementary to a single-stranded RNA (antisense transcript) having a base sequence complementary to mRNA of iNOS gene
Data Source
AI summary
The invention relates to sense oligonucleotide having a sequence complementary to a single-stranded RNA (antisense transcript) having a sequence complementary to mRNA of iNOS gene in order to control expression of iNOS (inducible nitric oxide synthase). The sense oligonucleotide of the present invention can control expression of iNOS and is useful for biological defense and treatment and prevention of diseases related to excessive production of NO, such as cancerogenesis, inflammatory disease, endotoxin shock by bacterial infection and the like.