Culture Vessel With Separate Supply and Discharge Ports for Cell Propagation
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Solution Overview
Problem
Conventional cell culture devices face challenges in efficiently culturing target cells, particularly induced pluripotent stem cells (iPS cells), as they struggle with the integration of cell generation, culturing, and propagation in a single device.
Innovation Solution
A culture vessel and cell culture device are designed with separate supply and discharge ports for liquids and a gas supply device to manage the flow paths independently, ensuring efficient cell culture by preventing residual liquid contamination and optimizing fluid circulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a single cell culture device is used for both cell generation and culturing, then device complexity is reduced, but culturing efficiency deteriorates
Solution Approach 1:
The cell culture device is segmented into multiple independent chambers: a first chamber for cell generation and a second chamber for cell culturing. This segmentation allows each chamber to be optimized for its specific function while maintaining a unified device structure, thereby reducing overall complexity without compromising culturing efficiency.
Solution Approach 2:
The cell culture device is designed with multi-functionality to perform both cell generation and culturing operations within a single integrated system. The device includes multiple chambers that can independently execute different functions (generation and culturing), allowing it to serve universal purposes while maintaining operational efficiency.
2Productivity
If separate supply and discharge ports are used, then fluid management efficiency is improved, but device complexity increases
Solution Approach 1:
The fluid management system is segmented into separate supply ports and discharge ports, allowing independent control of liquid inflow and outflow. This segmentation enables efficient fluid management by preventing contamination and allowing simultaneous operations without interfering with each other, while the modular design keeps overall device complexity manageable.
Data Source
AI summary
A culture vessel is arranged in a cell culture device and holds: a second cell generated from a first cell; and a culture medium, and the culture vessel includes: a supply port through which a liquid and the culture medium are supplied to the culture vessel, the liquid including the first cell and being from a cell vessel, the culture medium being from a culture medium vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.