Sequencing Fluidics Degassing to Prevent Flow Cell Bubbles
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Solution Overview
Problem
Gas present in sequencing reagents and nucleic acid samples forms bubbles in the flow cell, affecting image quality and sequencing accuracy in high-throughput gene sequencing.
Innovation Solution
A fluidic system with a degassing device connected to a pipeline to remove gas from liquid, using a sealed chamber under negative pressure to degas the liquid before it enters the reaction device, and a control method to ensure the degassing device functions properly.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If gas is present in sequencing reagents and nucleic acid samples, then the sequencing process can proceed normally, but bubbles form in the flow cell affecting image quality and sequencing accuracy
Solution Approach 1:
The degassing device performs preliminary removal of gas from the liquid before the liquid enters the flow cell. The system includes a degassing chamber where gas is extracted from the sequencing reagents and nucleic acid samples prior to they reaching the reaction site, preventing bubble formation during the sequencing process.
Solution Approach 2:
The patent extracts and removes gas from the liquid stream using a degassing device. The degassing chamber captures and separates gas bubbles from the liquid, allowing the gas to be removed while the degassed liquid continues to the flow cell, thereby eliminating the harmful bubbles that would otherwise form in the sequencing process.
2Reliability
If a degassing device is added to remove gas from liquid, then bubble formation is reduced, but device complexity increases
Solution Approach 1:
The degassing chamber is integrated into the existing fluidic system architecture, merging the gas removal function with the liquid delivery system. The degassing device is positioned inline with the fluidic pathways, allowing gas removal without requiring separate independent systems, thus minimizing the increase in overall device complexity.
Solution Approach 2:
The degassing chamber serves as an intermediary component between the liquid source and the flow cell. It mediates the interaction between gas and liquid by providing a controlled environment where gas is removed from the liquid stream, acting as a buffer that prevents gas bubbles from reaching the sequencing process while maintaining system simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Reduces bubble formation in the reaction device, improving sequencing quality by ensuring gas is removed from the liquid, thereby enhancing the accuracy and efficiency of the sequencing process.
Implementation Method 1
the portion of the pipeline located within the sealed chamber is capable of, when the sealed chamber is under a negative pressure condition, allowing the gas in the liquid in the pipeline to permeate out
Implementation Method 2
allowing the gas in the liquid in the pipeline to permeate out
Data Source
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AI summary
The present application discloses a fluidic system, a control method, a sequencing system, and a storage medium. The fluidic system includes a reaction device, a pipeline, and a degassing device. The reaction device is configured to provide a site for carrying out biochemical reactions. The pipeline is in communication with the reaction device and configured to deliver liquid to the reaction device and/or receive liquid flowing out from the reaction device. The degassing device is connected to the pipeline and configured to remove gas from the liquid in the pipeline. In this way, by removing gas from the liquid in the pipeline using the degassing device, the formation of bubbles in the reaction device can be reduced.