Sequencing Run Quality Monitoring With Real-Time Feedback Control
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Solution Overview
Problem
Current nucleic acid sequencing technologies are expensive, time-consuming, and generate large amounts of data, with limited real-time monitoring and control capabilities, leading to inefficiencies and potential errors in data quality.
Innovation Solution
A system where a sequencing instrument is in communication with a processor that monitors data, modifies operations, and provides information to operators, enabling real-time monitoring and control of sequencing runs, including comparison of sample and control data to ensure quality and adjust parameters such as reagent delivery and sequencing cycles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If real-time monitoring and control capabilities are added to sequencing instruments, then data quality monitoring is enhanced, but device complexity increases
Solution Approach 1:
The patent implements real-time monitoring of sequencing run quality parameters and feeds this information back to control the sequencing instrument. The system continuously monitors quality metrics during the sequencing run and automatically adjusts parameters or alerts operators when quality thresholds are not met, enabling closed-loop quality control without requiring complex manual intervention.
Solution Approach 2:
The patent introduces a quality control system that acts as an intermediary between the sequencing instrument and the operator. This intermediary layer automatically analyzes quality data, interprets results against predefined criteria, and communicates only essential information to operators, reducing the complexity burden on both the instrument and the user while maintaining comprehensive quality monitoring.
2Reliability
If run-time quality monitoring is implemented, then errors are detected earlier, but loss of time occurs during monitoring and intervention
Solution Approach 1:
The patent establishes quality thresholds and monitoring criteria before the sequencing run begins. By pre-configuring acceptance criteria and automated response protocols, the system can immediately compare real-time data against these predetermined standards without requiring time-consuming analysis during the run, enabling rapid error detection and response.
Solution Approach 2:
The system automatically monitors quality parameters, compares results against thresholds, and triggers appropriate responses without requiring continuous operator attention. The automated quality control system serves itself by independently analyzing data and initiating corrective actions or alerts, minimizing the time operators need to spend on manual monitoring while maintaining continuous quality oversight.
3Reliability
If continuous monitoring of sequencing runs is performed, then data quality is improved, but use of energy increases
Solution Approach 1:
The patent implements periodic sampling and monitoring of quality parameters at strategically selected intervals during the sequencing run rather than continuous uninterrupted monitoring. The system checks quality metrics at key checkpoints and uses predictive algorithms to assess overall run quality, maintaining high data quality assurance while significantly reducing the energy consumption associated with constant data acquisition and processing.
Data Source
AI summary
Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.