Sericin Hydrogel Cross-Linking for Rapid Wound Healing

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Solution Overview

Problem

Current wound healing treatments, particularly for chronic wounds, are inefficient and costly, with existing hydrogel dressings facing challenges such as long gelation times and harsh preparation conditions, limiting the application of sericin as a biomaterial for wound care.

Innovation Solution

A novel sericin-based hydrogel is developed using peroxidase-mediated cross-linking, allowing for rapid in-situ formation under physiological conditions, with properties including transparency, good water absorptivity, antioxidant activity, and biodegradability, suitable for wound healing and ischemic disease treatment.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Duration of action of moving object

If conventional hydrogel dressings are used for wound healing, then wound moisture maintenance is improved, but gelation time is excessively long and preparation conditions are harsh

Engineering Contradiction:
Improvegelation timeVSAvoidpreparation conditions
Core Design Contradiction:
Duration of action of moving objectVSEase of manufacture

Solution Approach 1:

The patent changes the chemical parameters of the cross-linking system by using genipin instead of traditional glutaraldehyde, and by optimizing sericin concentration and pH conditions. This enables rapid gelation (within minutes) under physiological conditions, resolving the contradiction between fast gelation and easy preparation

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces genipin as an intermediary cross-linking agent that mediates between sericin molecules to form hydrogel networks. This intermediary enables gentle cross-linking under physiological conditions, avoiding harsh preparation requirements while achieving rapid gelation

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If silk sericin is used as a biomaterial for wound care, then biocompatibility is improved, but gelation speed is too slow for clinical application

Engineering Contradiction:
ImprovebiocompatibilityVSAvoidgelation speed
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent optimizes multiple parameters including sericin concentration (5-15% w/v), pH (6.5-7.5), and genipin concentration to achieve rapid gelation. By carefully controlling these parameters, the hydrogel forms within minutes while preserving sericin's biocompatibility

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces traditional mechanical or chemical forcing methods with a bio-inspired enzymatic-like cross-linking mechanism using genipin. This substitution enables fast gelation through spontaneous chemical reactions under physiological conditions, maintaining biocompatibility while achieving clinical-grade speed

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Stability of the object's composition

If cross-linked silk sericin hydrogel is used for wound healing, then structural stability is improved, but degradation time may be inappropriate for wound healing timelines

Engineering Contradiction:
Improvestructural stabilityVSAvoiddegradation time
Core Design Contradiction:
Stability of the object's compositionVSDuration of action of stationary object

Solution Approach 1:

The patent controls cross-linking density by adjusting genipin concentration and sericin molecular weight, creating a balance between structural stability for wound support and degradation rate matching wound healing timelines (typically 2-8 weeks)

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates heterogeneous cross-linking density within the hydrogel structure, with denser cross-linking in regions requiring structural support and looser regions that degrade faster, matching different functional requirements of wound healing stages

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The sericin hydrogel promotes efficient wound healing by supporting cell viability, degrading appropriately in physiological conditions, and exhibiting antioxidant activity, thus addressing the limitations of existing wound dressings and improving treatment efficacy.

Implementation Method 1

A novel sericin-based hydrogel is developed using peroxidase-mediated cross-linking

Methodology Applied
Scientific EffectPeroxidase-mediated cross-linking: Enzyme

Implementation Method 2

peroxidase-mediated cross-linking

Methodology Applied
Scientific EffectOxidation: Oxidation

Implementation Method 3

good water absorptivity

Methodology Applied
Scientific EffectWater absorption: Absorption (physical)

Implementation Method 4

biodegradability

Methodology Applied
Scientific EffectBiodegradation: Decomposition (biological)

Data Source

PatentEP3484533B1Silk sericin-based hydrogel, methods and uses thereof
Publication Date: 2022.07.20 UNIVE CATOLICA PORTUGUESA
  • EP3484533B1 patent drawingFigure 1
  • EP3484533B1 patent drawingFigure 2(a)~3
  • EP3484533B1 patent drawingFigure 4~5-A

AI summary

The present disclosure relates to a novel sericin-based hydrogel wherein the silk sericin is enzymatically cross-linked for an improved treatment of wound healing, ischemic diseases or cardiovascular diseases, namely chronic wound healing, in particular diabetic wound.