Serum-Free Cryopreservation Medium for Adipose-Derived Stem Cells

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Solution Overview

Problem

Current cryopreservation methods for adipose-derived stem cells rely on serum, which is costly, variable in quality, and can be toxic, making it essential to develop a serum-free freezing medium for effective long-term preservation and establishment of adipose-derived stem cell libraries.

Innovation Solution

A serum-free freezing medium comprising serum-free culture medium, dimethylsulfoxide (DMSO), and Knockout™ Serum Replacement (KSR) is developed, with specific volume ratios optimized for effective cryopreservation, ensuring high cell survival and differentiation potential.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If serum is used in freezing medium, then cell protective effect is improved, but cell toxicity increases due to polyamine oxidase and other harmful substances

Engineering Contradiction:

Improvecell protective effectVSAvoidcell toxicity

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and removes the harmful components (polyamine oxidase, complement, antibodies, bacteria toxins) from the serum by using serum-free culture medium combined with specific protective additives, thereby eliminating cell toxicity while preserving the protective function

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent introduces intermediary substances (protective additives, growth factors, trace elements) that mediate the protective function previously provided by serum, replacing the harmful serum composition with a safer alternative mixture

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If animal serum is used in freezing medium, then cell growth support is improved, but batch consistency deteriorates due to individual animal variation

Engineering Contradiction:

Improvecell growth supportVSAvoidbatch consistency

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent creates a standardized copy of serum's beneficial functions through defined components (serum-free medium with specific growth factors and trace elements), eliminating the variability inherent in animal-derived serum batches

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent changes the composition parameters from variable animal serum to a controlled, defined mixture of serum-free medium, protective additives, and standardized growth factors, ensuring consistent results across batches

Inventive Principle:

Principle #35Parameter changes

3Productivity

If serum is used in freezing medium, then cell proliferation is improved, but contamination risk increases due to mycoplasma and viruses

Engineering Contradiction:

Improvecell proliferationVSAvoidcontamination risk

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent removes the contamination risk by extracting and eliminating serum from the freezing medium, using instead a serum-free formulation that does not harbor mycoplasma or viruses while maintaining cell proliferation support through alternative components

Inventive Principle:

Principle #2Taking out (Extraction)

4Reliability

If serum is used in freezing medium, then cell culture function is improved, but cost increases to 3000-4000 RMB per 500 ml

Engineering Contradiction:

Improvecell culture functionVSAvoidcost

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The patent replaces expensive serum with a more cost-effective serum-free medium formulation using readily available components (protective additives, growth factors, trace elements) that achieve the same cell culture function at lower cost

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The serum-free medium provides superior cryopreservation results, with adipose-derived stem cells maintaining high viability and differentiation ability post-thawing, overcoming the limitations of traditional serum-based methods.

Implementation Method 1

Methodology Applied

Scientific EffectCryoprotection:

Implementation Method 2

The serum-free medium provides superior cryopreservation results, with adipose-derived stem cells maintaining high viability and differentiation ability post-thawing

Methodology Applied

Scientific EffectCryopreservation:

Data Source

PatentUS9481865B2Serum-free freezing medium used in adipose-derived stem cells and establishment of adipose-derived stem cell library

Publication Date: 2016.11.01 CELLULAR BIOPHARMA (SHANGHAI) LTD

AI summary

Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity.