Serum-Free Tissue Media for Storage, Irrigation, and Infusion

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Solution Overview

Problem

Existing storage and irrigation solutions for cells and tissues in healthcare and veterinary settings are inadequate, leading to cell death due to air drying, osmotic imbalance, and exposure to harmful media, which compromises tissue viability and increases infection risk.

Innovation Solution

The use of serum-free and growth factor-free tissue culture media, such as Dulbecco's Modified Eagle Medium (DMEM) and alpha-minimum essential medium (aMEM), for temporary storage and irrigation of tissues and cells, which maintains cell viability and integrity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Duration of action of moving object

If cells and tissue are stored outside of the body, then temporary storage is enabled, but cell and tissue survival is compromised due to environmental and biological condition changes

Engineering Contradiction:

Improvetemporary storage durationVSAvoidcell and tissue survival

Core Design Contradiction:

Duration of action of moving objectVSReliability

Solution Approach 1:

The patent changes the chemical and physical parameters of the storage environment by using a serum-free tissue culture medium with specific composition (amino acids, vitamins, minerals, buffering agents) to maintain cell viability during temporary storage outside the body

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent introduces a specialized tissue culture medium as an intermediary substance between the cells/tissue and the external environment, which mediates the storage process by providing necessary nutrients and protective conditions while enabling temporary storage

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of operation

If conventional irrigation media are used, then wound irrigation is performed, but tissue damage and infection risk increase

Engineering Contradiction:

Improvewound irrigation capabilityVSAvoidtissue damage and infection risk

Core Design Contradiction:

Ease of operationVSObject-affected harmful factors

Solution Approach 1:

The patent changes the chemical composition parameters of the irrigation medium from conventional crystalloids to a serum-free tissue culture medium with specific amino acids, vitamins, and buffering agents, which reduces tissue damage and infection risk while maintaining irrigation functionality

Inventive Principle:

Principle #35Parameter changes

3Reliability

If serum-containing media are used for storage, then cell viability is maintained, but biological characteristics are altered

Engineering Contradiction:

Improvecell viabilityVSAvoidbiological characteristics

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent extracts and removes serum from the storage medium composition, using instead a defined serum-free tissue culture medium that maintains cell viability through alternative nutrients and growth factors, thereby preventing alteration of biological characteristics

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes the compositional parameters by replacing serum with specific concentrations of amino acids, vitamins, minerals, and buffering agents, which maintains cell viability while preserving biological characteristics through a controlled, defined composition

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS20250281271A1Tissue storage, irrigation, and infusion medium and methods of use

Publication Date: 2025.09.11 NEW YORK SOC FOR THE RUPTURED & CRIPPLED MAINTAINING THE HOSPITAL FOR SPECIAL SURGERY

AI summary

This disclosure features tissue storage, irrigation, and infusion media compositions, and methods of using such compositions, for example, the temporary storage of cells and tissues prior to implantation or re-implantation, or for wound irrigation, wound packing, surgical site irrigation, or intravenous infusion. The tissue storage, irrigation, and infusion media compositions disclosed herein are useful in a healthcare or veterinary setting.