Setosphaeria monoceras Mycoherbicidal Composition for Water Hyacinth Control

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Solution Overview

Problem

Current methods for controlling water hyacinth, a noxious aquatic weed, are ineffective due to its reproductive capacity and resistance to environmental stress, and synthetic chemical herbicides pose environmental and health concerns, while bioherbicides are unstable under temperature and UV exposure.

Innovation Solution

A novel herbicidal composition using Setosphaeria monoceras strain AGWH#11, deposited as MTCC 5974 and NCIM 1370, which includes cell-free culture filtrate and purified phytotoxins, formulated with agriculturally acceptable carriers to maintain viability and effectiveness, providing a host-specific biocontrol agent for water hyacinth.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If synthetic chemical herbicides are used to control water hyacinth, then immediate weed control is achieved, but environmental and health concerns arise due to toxic effects

Engineering Contradiction:
Improveweed control effectivenessVSAvoidenvironmental and health toxicity
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effects of chemical herbicides into beneficial biological control by using fungal pathogens (Setosphaeria monoceras) that naturally infect and kill water hyacinth. The fungus produces phytotoxins that selectively target the weed while being environmentally benign, thus converting the problem of chemical toxicity into a solution using biological agents.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent introduces a fungal intermediary (Setosphaeria monoceras) that mediates between the desired weed control function and environmental safety. The fungus acts as a biological mediator that delivers herbicidal activity through natural pathogenic mechanisms, avoiding direct application of synthetic chemicals while maintaining effective weed suppression.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Object-affected harmful factors

If bioherbicides are used to control water hyacinth, then environmental safety is improved, but stability under temperature and UV exposure deteriorates

Engineering Contradiction:
Improveenvironmental safetyVSAvoidstability under temperature and UV
Core Design Contradiction:
Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent applies parameter changes by formulating the bioherbicide with protective agents that modify the physical and chemical environment of the fungal spores. The formulation includes carriers and adjuvants that change the stability parameters of the bioherbicide, enabling it to withstand temperature fluctuations and UV radiation while maintaining biological activity.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite bioherbicide formulation that combines fungal spores with protective carriers, surfactants, and stabilizing agents. This composite structure protects the biological agent from environmental stressors while maintaining its herbicidal function, solving the stability issue without compromising environmental safety.

Inventive Principle:
Principle #40Composite materials

3Adaptability or versatility

If fungal biocontrol agents are used to control water hyacinth, then host specificity is improved, but large-scale production and formulation difficulty increases

Engineering Contradiction:
Improvehost specificityVSAvoidlarge-scale production and formulation
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent extracts the active herbicidal components from the fungal system, utilizing cell-free culture filtrates and purified phytotoxins. This extraction approach separates the harmful metabolic products from the complex fungal biology, simplifying production and formulation while maintaining host-specific activity through the selective action of the isolated toxins.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates simplified copies of the fungal herbicidal action by producing and applying purified phytotoxins and culture filtrates without the need for live fungal cultures. This copying approach replicates the biological control effect while eliminating the complexities of fungal cultivation, storage, and formulation.

Inventive Principle:
Principle #26Copying

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Setosphaeria monoceras-based composition effectively reduces water hyacinth biomass and demonstrates high herbicidal activity, offering a stable and environmentally friendly alternative to synthetic herbicides, with potential for repeated applications and integration with other control measures.

Implementation Method 1

The isolate's spores, cell free culture filtrate, crude metabolites and pure purified phytotoxin obtained therefrom, useful for the control of potential biological control agent for water hyacinth

Methodology Applied
Scientific EffectPhytotoxin production:

Data Source

PatentUS10206405B2Mycoherbicidal composition for suppressing water hyacinth
Publication Date: 2019.02.19 LAXMINARAYANA PRAHARAJU
  • US10206405B2 patent drawing
  • US10206405B2 patent drawing
  • US10206405B2 patent drawing

AI summary

The present invention deals with a mycoherbicidal isolate of Setosphaeria monoceras sp. or extracts obtained therefrom, useful for the control of Water hyacinth. The present invention also discloses biological control compositions comprising fungal isolate extract formulated in a growth medium for maintaining the viability of extract when the biological control composition is applied to weed. The present invention also discloses methods of screening fungal isolate to determine if they exhibit biocontrol activity. The present invention also discloses use of Setosphaeria monoceras strain. In yet a further aspect the invention relates to use of an Setosphaeria strain for producing a herbicidal agent effective for controlling growth of water hyacinth plants. The strain used preferably is a strain having the characterizing features of Setosphaeria strain AGWH#1 1 as deposited at IMTECH under accession number MTCC 5974. The present invention to provide the 18s rRNA sequence analysis showed 96% nucleotide similarity of the Fungi to Setosphaeria monoceras. (AGBIO designated AGWH#1 1/NCIM1370/MTCC 5974) but also indicated nucleotide variation of this fungus from other known genus of Bipolaris, Pleosporaceae, Cochiliobolus, Alternaria including an environmentally acceptable alternative to synthetic chemical herbicides for the control of weeds, such as Water hyacinth. The present invention also discloses methods of whole genome study of strain.