Sheep SNP Molecular Marker for Brucellosis Resistance Detection
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Solution Overview
Problem
Current breeding methods for sheep lack an effective molecular marker to detect resistance to brucellosis, a zoonotic disease causing significant economic losses and public health risks.
Innovation Solution
A sheep single nucleotide polymorphism (SNP) molecular marker associated with resistance to brucellosis is identified through a genome-wide association study (GWAS), with a polymorphic site at position 35,704,228 of chromosome 3, showing a T/C polymorphism. This marker is used in conjunction with a primer combination for efficient detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional breeding methods are used for sheep, then breeding processes are simple and easy to operate, but breeding efficiency is low and breeding time is long
Solution Approach 1:
The patent introduces SNP molecular markers as an intermediary tool between traditional breeding and disease resistance outcomes. These markers serve as mediators that enable selective breeding for brucellosis resistance without requiring complex disease challenge studies or lengthy observation periods, thus improving breeding efficiency while maintaining operational simplicity
Solution Approach 2:
The patent applies preliminary action by using molecular markers to identify and select breeding stock with desired resistance traits before actual breeding occurs. This advance identification through SNP marker analysis allows planners to pre-select parents with favorable genotypes, eliminating the need for lengthy post-breeding evaluation periods and significantly reducing breeding time
2Loss of time
If molecular marker-assisted breeding is implemented, then breeding efficiency is improved and breeding time is reduced, but detection requirements and technical complexity increase
Solution Approach 1:
The patent extracts the specific SNP marker sequences associated with brucellosis resistance from the complex sheep genome. By isolating and characterizing these specific molecular markers (such as the markers identified in chromosomes 1, 3, 6, 10, 13, 16, 19, 20, 21, and 27), the patent converts an otherwise intractable genome-wide analysis problem into a simple, targeted detection task using PCR-based methods
Solution Approach 2:
The patent creates simplified copies of the resistance information by developing PCR primers that amplify specific SNP marker regions. These molecular copies serve as proxies for the actual resistance traits, allowing rapid identification of resistant animals through simple PCR amplification and detection of the marker sequences rather than requiring complex genomic analysis or disease challenge studies
3Measurement precision
If no molecular markers are used, then detection methods are simple, but resistance level identification is inaccurate and breeding precision is low
Solution Approach 1:
The patent applies local quality by focusing detection efforts on specific, localized SNP marker positions within the genome that are known to be associated with brucellosis resistance. Rather than attempting to measure or analyze the entire genome or multiple unrelated traits, the patent identifies and targets specific chromosomal locations (such as positions on chromosomes 1, 3, 6, 10, 13, 16, 19, 20, 21, and 27) where resistance-determining variants occur, thereby achieving high measurement precision through targeted analysis of specific genomic regions
Data Source
AI summary
A sheep single nucleotide polymorphism (SNP) molecular marker and use thereof in detecting resistance to brucellosis in sheep are provided, relating to the technical field of molecular marker-assisted breeding. A sheep SNP molecular marker is provided, where the sheep SNP molecular marker is associated with resistance to brucellosis in sheep, and has a nucleotide sequence shown in SEQ ID NO: 1 in which a T/C polymorphism is located at 101st bp. The sheep SNP molecular marker can accurately detect a resistance level of sheep to the brucellosis, and is used for the selection of brucellosis-resistant sheep as well as molecular marker-assisted breeding of the resistance to brucellosis in sheep. The sheep SNP molecular marker effectively improves a breeding efficiency of sheep with brucellosis resistance.