Sheet-Shaped Cell Culture Bag Assembly for Aseptic Preparation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The introduction of large-scale equipment for maintaining aseptic conditions during sheet-shaped cell culture preparation is costly and limits the use of this technology to specialized facilities, necessitating a more accessible and cost-effective solution.
Innovation Solution
A device comprising interconnected bags and filters for aseptic preparation of sheet-shaped cell cultures, allowing for mixing, washing, and culturing without large-scale equipment, using a support for compact storage and transport.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If large-scale equipment such as an isolator is introduced to reduce contamination risk, then the reliability of cell culture preparation is improved, but the device complexity and capital investment costs increase
Solution Approach 1:
The device divides the cell culture preparation system into multiple separate functional bags (cell suspension bag, diluent bag, medium bag, culture bag) that can be independently prepared and connected through fluid pathways. This segmentation allows each bag to be sterilized and handled separately, reducing the need for large-scale isolators while maintaining aseptic conditions.
Solution Approach 2:
The invention introduces a mixing bag as an intermediary component that receives cell suspension, diluent, and medium from separate bags, mixes them aseptically, and transfers the mixture to the culture bag. This intermediary approach enables controlled mixing without direct contact between multiple open containers, reducing contamination risk without requiring large-scale equipment.
2Reliability
If large-scale equipment is introduced to maintain aseptic conditions, then the reliability of cell culture preparation is improved, but the ease of operation and accessibility to specialized facilities worsen
Solution Approach 1:
By dividing the system into separate pre-sterilized bags that can be connected through standardized fluid pathways, the invention enables aseptic preparation in facilities without large-scale isolators. Each bag can be prepared independently and connected in a controlled manner, making the system accessible to常规 facilities.
Solution Approach 2:
The invention uses disposable sterile bags instead of expensive, complex large-scale equipment. Each bag is pre-sterilized and used once, then discarded, eliminating the need for complex sterilization systems and large-scale isolators while maintaining aseptic conditions. This makes the system economically accessible to more facilities.
3Manufacturing precision
If centrifugal separators are used for cell processing, then the manufacturing precision of cell separation is improved, but the device complexity and cost increase
Solution Approach 1:
The invention extracts the cell separation function from complex centrifugal separator equipment and achieves it through simple filtration using a filter bag. The filter bag separates cells from the medium through filtration principles, eliminating the need for expensive centrifugal equipment while maintaining effective cell separation.
Solution Approach 2:
The invention replaces the mechanical centrifugal separation system with a filtration-based separation system. Instead of using centrifugal force generated by rotating machinery, the system uses passive filtration through a filter bag to separate cells from the medium, significantly reducing device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables aseptic preparation and handling of sheet-shaped cell cultures with reduced contamination risk, improved cell survival rates, and efficient transport and storage, eliminating the need for centrifugal separators.
Implementation Method 1
a filter being fluidly connected to the mixing bag
Data Source
AI summary
A device including a mixing bag for mixing liquids, a cell-suspension bag fluidly connected to the mixing bag, a diluent bag fluidly connected to the mixing bag, and a fluidly connected to the mixing bag is capable of easily preparing a sheet-shaped cell culture without requiring any large-scale equipment. The sheet-shaped cell culture can be aseptically prepared by the device, while reducing the risk of contamination, by using the mixing bag which is fluidically connected to the other bags. The device includes a support having a foldable structure. At least one of the cell-suspension bag, the diluent bag and the filter is fixed to various portions of the support. The device can be folded into a compact size, by folding the various portions of the support, allowing for efficient transportation and storage.