Shield Layer Culture Medium Device for Polyporales
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Solution Overview
Problem
Conventional culture medium devices for cultivating Polyporales suffer from microbiological contamination due to heterogeneous populations, leading to lower yields and increased costs associated with maintaining clean rooms.
Innovation Solution
A culture medium device with a shield layer containing select components such as sugar, agar, inorganic agar, glycerol, vegetable oil, or plant essential oils, applied at specific concentrations to reduce contamination and promote biomass productivity by inhibiting the growth of heterogeneous populations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Use of energy by moving object
If a conventional culture medium device with a gap between lid and plate is used for ventilation, then airflow and oxygen supply are improved, but heterogeneous population contamination increases
Solution Approach 1:
A shield layer is introduced as an intermediary substance between the culture medium and the external environment. This shield layer selectively allows oxygen and carbon dioxide to pass through while blocking spores and heterogeneous populations, thus mediating the contradiction between ventilation needs and contamination prevention
Solution Approach 2:
The shield layer is composed of composite materials with specific properties: sugar concentration (0.1-10%), agar (0.1-5%), and optional additives like vegetable oil or plant essential oils. This composite structure creates a selective barrier that permits gas exchange while preventing microbial contamination
2Object-affected harmful factors
If clean room environment is used to reduce contamination, then heterogeneous population damage is reduced, but cost increases greatly
Solution Approach 1:
The culture medium device becomes self-protecting through the shield layer, which inherently prevents contamination without requiring external clean room infrastructure. The shield layer autonomously filters out spores and heterogeneous populations while allowing necessary gas exchange, making the system self-sufficient in preventing contamination
Solution Approach 2:
The shield layer uses inexpensive, easily replaceable materials such as sugar, agar, and plant essential oils that can be prepared and disposed of economically, replacing the need for expensive clean room facilities
3Object-affected harmful factors
If shield layer with sugar and agar is applied on culture medium surface, then heterogeneous population growth is inhibited, but device complexity increases
Solution Approach 1:
The shield layer's effectiveness is controlled by adjusting parameters such as sugar concentration (0.1-10%), agar concentration (0.1-5%), and thickness (1 μm-1 cm). These parameter variations allow optimization of anti-contamination performance while maintaining simplicity in the basic device structure
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The shield layer effectively decreases microbiological contamination, resulting in higher yields and increased productivity of Polyporales biomass by making it difficult for spores of bacteria and heterogeneous populations to grow, as demonstrated in experiments with various strains like Ganoderma lucidum and Phellinus linteus.
Implementation Method 1
The shield layer effectively decreases microbiological contamination, resulting in higher yields and increased productivity of Polyporales biomass by making it difficult for spores of bacteria and heterogeneous populations to grow
Data Source
AI summary
The present invention discloses a culture medium device, which comprises a plate containing a solid-state or gel-state culture medium layer; a shield layer covering the culture medium layer; and a lid covering the plate. The shield layer is in a solid, gel or liquid state and contains sugar at a concentration of greater than 0% (w/w) and less than or equal to 0.1% (w/w). Via the shield layer containing a select composition, the present invention can reduce the affection of microbiological contamination and promote the yield and productivity of biomass in the process of inoculating and cultivating Polyporales.