Side Wall Reaction Field for Detection Chip Surface Interference
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Solution Overview
Problem
The existing detection systems face challenges in maintaining detection accuracy due to residual liquid in the well and interference from the liquid surface during the detection process, particularly when the inner diameter of the well is small or when bubbles are present on the liquid surface.
Innovation Solution
A detection chip and system design where the reaction field is arranged on the side wall of the housing, allowing for efficient liquid removal and minimizing the impact of the liquid surface on detection results, with a light source generating evanescent light or surface plasmon resonance to detect the substance without passing through the liquid surface.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If the reaction field is arranged on the bottom surface of the well, then the detection system can be simplified, but the liquid cannot be sufficiently removed and remaining liquid deteriorates detection accuracy
Solution Approach 1:
The reaction field is relocated from the bottom surface (vertical dimension) to the side wall (horizontal dimension) of the well. This dimensional change allows the liquid transfer device tip to contact the bottom surface for complete liquid removal, while the reaction field on the side wall remains accessible to the analyte. The side wall arrangement enables both complete liquid removal and maintained detection accuracy.
2Device complexity
If the detection unit is arranged above the well to detect fluorescence through the liquid surface, then the detection system can be simplified, but the detection result is affected by the liquid surface and bubbles
Solution Approach 1:
The detection approach transitions from detecting fluorescence through the liquid surface (vertical path) to detecting fluorescence from the side wall reaction field (horizontal path). By arranging the reaction field on the side wall, the fluorescence detection path is modified to avoid passing through the liquid surface, thereby eliminating interference from surface effects and bubbles while maintaining system simplicity.
3Volume of stationary object
If the inner diameter of the well is made small to reduce volume, then the well volume is reduced, but the detection result is affected by the meniscus
Solution Approach 1:
The reaction field is positioned on the side wall rather than at the bottom surface, changing the spatial dimension of the reaction zone. This allows the well to maintain a small inner diameter for volume reduction while the side wall reaction field avoids the meniscus region, ensuring accurate detection results are not affected by surface tension effects.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This design enhances detection accuracy by ensuring complete liquid removal and reducing surface interference, leading to reliable detection of substances with improved precision.
Implementation Method 1
a light source that irradiates the detection chip with light from the outside such that evanescent light is generated on the inner surface of the side wall at a position corresponding to the reaction field
Implementation Method 2
surface plasmon resonance is generated in the metal film
Implementation Method 3
the fluorescent substance is excited by an electric field enhanced by surface plasmon resonance (hereinafter also referred to as 'SPR') and emits fluorescence
Data Source
AI summary
The detection system according to the present invention has a detection chip, a light source, and a detection unit. The detection chip has a housing that has an opening at an upper portion, and a reaction field for trapping a substance to be detected, the reaction field being arranged on an inner surface of the side walls included in the housing. The light source irradiates the detection chip with light from the outside such that evanescent light or surface plasmon resonance is generated under the reaction field. The detection unit detects light which is emitted from the detection chip when the light source irradiates the detection chip with light, and the amount of which changes depending on the amount of the substance to be detected trapped in the reaction field.