Sidwf1 Gene Controls Sesame Internode Length

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Solution Overview

Problem

Current sesame varieties with tall stems are not suitable for high-density cultivation and mechanical harvesting due to their internode length, limiting breeding efficiency and crop management.

Innovation Solution

Identification and utilization of the Sidwf1 gene, which controls internode length in sesame, along with specific SNP markers for PCR amplification and phenotypic differentiation, enabling the breeding of sesame varieties with short internode length and improved cultivation practices.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Length of moving object

If sesame varieties with tall stems are cultivated, then the plant height is increased, but the internode length becomes too long for high-density cultivation and mechanical harvesting

Engineering Contradiction:

Improveinternode lengthVSAvoidbreeding efficiency

Core Design Contradiction:

Length of moving objectVSProductivity

Solution Approach 1:

The patent identifies and utilizes the Sidwf1 gene to change the internode length parameter in sesame varieties. By applying molecular breeding techniques targeting this specific gene, the invention achieves precise modification of the internode length parameter to fall within the optimal range of 3.8-4.6 cm, resolving the contradiction between plant height and cultivability.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent replaces traditional mechanical selection methods with molecular marker-assisted breeding. By using SNP markers linked to the Sidwf1 gene, breeders can identify and select for desired internode length phenotypes at the molecular level, significantly improving breeding efficiency and enabling precise control over the internode length parameter.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Loss of time

If traditional breeding methods are used to select for short internode length, then the selection process is time-consuming, but molecular markers enable faster and more accurate selection

Engineering Contradiction:

Improvebreeding timeVSAvoidphenotype screening accuracy

Core Design Contradiction:

Loss of timeVSMeasurement precision

Solution Approach 1:

The patent introduces molecular markers (SNP markers) as intermediaries between the Sidwf1 gene and the observable phenotype. These markers serve as reliable indicators that allow breeders to screen for the desired short internode length phenotype at the DNA level, eliminating the need for time-consuming growth trials and providing accurate prediction of the target phenotype.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent enables preliminary selection at the seedling stage using molecular markers. By detecting the presence of the Sidwf1 gene variant before the plant reaches maturity, breeders can identify and select promising genotypes early in the breeding process, dramatically reducing the time required to develop new varieties with the desired internode length.

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Sidwf1 gene provides a 100% explanation rate for the dwarf phenotype with short internode length, facilitating the development of new sesame varieties suitable for high-density cultivation and mechanical harvesting, enhancing breeding efficiency and understanding growth mechanisms.

Implementation Method 1

A mutation occurred at the 448th nucleotide of the wild-type (wt.) gene (that is: the gene controls normal internode length), i.e., the base is mutated from C (wt.) to T (dwf)

Methodology Applied

Scientific EffectGene mutation:

Implementation Method 2

amplifying the Sidwf1 gene or the allele SiDWF1 with the sesame genomic DNA extracted in (1) as a template with the primer pair

Methodology Applied

Scientific EffectPCR amplification:

Implementation Method 3

The following single nucleotide polymorphism (SNP) primers are used to distinguish the Sidwf1 gene and the allele SiDWF1

Methodology Applied

Scientific EffectSingle nucleotide polymorphism (SNP):

Data Source

PatentUS11519041B2<i>Sidwf1 </i>gene controlling internode length in sesame

Publication Date: 2022.12.06 HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI

AI summary

A Sidwf1 gene of Sesamum indicum, including two exons and an intron, is 1638 bp in total, and has a sequence represented by SEQ ID NO: 1. Also provided is a method for determining the internode length type in sesame samples, the method including: 1) extracting a genomic DNA of a sesame sample; 2) synthesizing three primers including SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R; amplifying the Sidwf1 gene or an allele SiDWF1 thereof with the genomic DNA of the sesame sample as a template, with a combination of SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R a combination of SiSNPdwf1 F1 and SiSNPdwf1 R, or a combination of SiSNPdwf1 F2 and SiSNPdwf1 R, as primers, thereby yielding a PCR product; and performing electrophoresis on the PCR product or sequencing the PCR product, and determining the phenotype of the sesame sample according to an electrophoresis or sequencing result.