Silver Nanoparticle Synthesis on DNA via 2-Mercapto-Thioxanthone

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Solution Overview

Problem

Current methods for synthesizing silver nanoparticles on DNA and BSA using long-duration UV illumination lead to deterioration of DNA and BSA, affecting the stability of silver nanoparticles and requiring an improvement for enhanced efficiency and stability.

Innovation Solution

The use of 2-mercapto-thioxanthone as a stabilization and photo-reducer agent to achieve the rapid reduction of silver salt to silver nanoparticles and nano-clusters in a short photo-illumination duration of 1 second, utilizing UV-Vis absorption and fluorescence emission spectra to monitor the formation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If long-duration UV illumination is used to synthesize silver nanoparticles on DNA and BSA, then silver nanoparticles can be formed, but DNA and BSA deteriorate and nanoparticle stability is reduced

Engineering Contradiction:
Improvenanoparticle formationVSAvoidnanoparticle stability
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent applies preliminary action by introducing 2-mercapto-thioxanthone as a stabilizing agent before UV illumination. This agent pre-establishes protective interactions with both DNA/BSA and silver ions, creating a stabilized complex that prevents deterioration during the subsequent rapid reduction process. The stabilizer is present in the system before the harmful UV exposure occurs, preventing the deterioration that would otherwise happen during nanoparticle formation.

Inventive Principle:
Principle #10Preliminary action

2Productivity

If long-duration UV illumination is used to synthesize silver nanoparticles, then nanoparticles can be produced, but the synthesis time is extended and efficiency is reduced

Engineering Contradiction:
Improvenanoparticle productionVSAvoidillumination duration
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies parameter changes by modifying the chemical environment through the addition of 2-mercapto-thioxanthone, which alters the reduction kinetics. This stabilizer enables the UV-induced reduction of silver ions to occur much more rapidly, allowing nanoparticle formation to be completed in approximately 1 second instead of requiring prolonged illumination. The chemical parameter change catalyzes the reduction process, dramatically缩短ing the synthesis time while maintaining nanoparticle quality.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If 2-mercapto-thioxanthone is used as photo-reducer agent, then synthesis speed and dimensional stability increase, but additional chemicals are introduced to the system

Engineering Contradiction:
Improvesynthesis speedVSAvoidchemical system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies the intermediary principle by using 2-mercapto-thioxanthone as a mediating substance that facilitates the interaction between UV light, silver ions, and the DNA/BSA template. This photo-reducer agent absorbs UV energy and transfers it to silver ions, enabling rapid reduction while simultaneously stabilizing the nanoparticle structure. The intermediary substance enables the desired transformation without requiring direct exposure of DNA/BSA to harsh UV conditions, thus achieving high synthesis speed with maintained stability.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method increases the synthesis speed and dimensional stability of silver nanoparticles, allowing for their formation in a dimension-controlled manner with reduced environmental impact and improved stability, suitable for diagnostic and treatment applications.

Implementation Method 1

photo-chemical synthesis and characterization of silver nanoparticles self-arranged on ct-DNA and BSA

Methodology Applied
Scientific EffectPhoto-chemical synthesis: Photopolymerisation

Implementation Method 2

Photovulcanization has some advantages over vulcanization like rapid curing, production at room temperature or at the minimum temperature

Methodology Applied
Scientific EffectPhoto-vulcanization: Photopolymerisation

Implementation Method 3

reduction of silver salt to silver nanoparticles by means of 2-mercapto-thioxanthone on DNA and BSA in short photo-illumination duration of 1 second

Methodology Applied
Scientific EffectPhoto-reduction: Photopolymerisation

Implementation Method 4

utilizing UV-Vis absorption and fluorescence emission spectra to monitor the formation

Methodology Applied
Scientific EffectAbsorption spectroscopy: Absorption Spectroscopy

Implementation Method 5

utilizing UV-Vis absorption and fluorescence emission spectra to monitor the formation

Methodology Applied
Scientific EffectFluorescence: Fluorescence

Data Source

PatentUS11565320B2Photo-chemical synthesis and characterization of silver nanoparticles self-arranged on ct-DNA and BSA
Publication Date: 2023.01.31 YILDIZ TEKNIK UNIVSI

AI summary

The present invention relates to examination of characteristics of silver nano-clusters self-arranged by means of reduction of silver salt to silver nanoparticles by means of 2-mercapto-thioxanthone on DNA and BSA in short photo-illumination duration of 1 second.