Simultaneous Raman and Light Scattering Detection Apparatus
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Solution Overview
Problem
Current detection apparatuses are unable to simultaneously detect Raman and light scattering from the same sample, limiting the analysis of molecular and size changes in nano materials, such as growth, binding between nanomaterials and proteins, and reactions between antigens and antibodies.
Innovation Solution
A simultaneous detection apparatus that applies incident light to a sample, detecting Raman scattering in specific geometries (180° or 90°) and light scattering in corresponding geometries, using filters and a computer to separate and analyze both types of scattering, allowing for the collection of information on particle size, distribution, and molecular structure.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If separate detection apparatuses are used for Raman scattering and light scattering, then each type of scattering can be detected individually, but simultaneous detection of both scattering types from the same sample is not achieved
Solution Approach 1:
The patent combines Raman scattering detection and light scattering detection into a single integrated apparatus. The detection unit includes both a Raman scattering detector with frequency shift detection capability and a light scattering detector with angle-based size measurement capability, allowing simultaneous detection of both scattering types from the same sample volume using a single incident light source.
Solution Approach 2:
The detection apparatus is designed with multi-functional capability to perform both Raman scattering detection (for molecular structure information) and light scattering detection (for particle size and distribution) using the same incident light source and detection unit, making the apparatus versatile for analyzing multiple sample characteristics simultaneously.
2Measurement precision
If Raman scattering detection is performed, then molecular vibrational structure information is obtained, but elastic scattered light must be removed which reduces detection efficiency
Solution Approach 1:
The patent uses frequency shift as an intermediary to distinguish Raman scattered light from elastic scattered light. The Raman scattering detector is designed to detect only the frequency-shifted scattered light, effectively filtering out the intense elastic scattered light without requiring additional removal devices, thus maintaining detection efficiency while obtaining molecular structure information.
3Measurement precision
If light scattering detection is performed with small collection angle, then particle size measurement accuracy is improved, but Raman scattering detection becomes more difficult due to geometric constraints
Solution Approach 1:
The detection unit is segmented into distinct detection channels: one optimized for light scattering detection with small collection angle for accurate particle size measurement, and another for Raman scattering detection with appropriate geometric configuration. This segmentation allows each detection function to operate at its optimal geometry without interfering with the other.
Data Source
AI summary
Provided is a detection apparatus of Raman scattering and light scattering, and more particularly, a simultaneous detection apparatus of Raman scattering and dynamic light scattering and a detection method using the same. The simultaneous detection apparatus of Raman scattering and light scattering includes: a detection unit for applying incident light to a sample, and detecting Raman scattering in 90° or 180° geometry and light scattering in 90° or 180° geometry in order to simultaneously collect Raman scattering and light scattering; and a computer connected to the detection unit to obtain at least one of the size and distribution of particles from the detected light scattering, and to obtain information of the molecular structure from the detected Raman scattering. This apparatus may simultaneously observe the size of nano-sized or larger material and molecular information thereof, and phenomena accompanying changes in molecular environment according to material variation and changes of the material in size and distribution, and thus is very useful for studying nano materials and protein antigens and antibodies.