Single Cell Compartment System for Integrated Imaging and Genome Detection
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Solution Overview
Problem
Current methods for detecting nondestructive measurement information and genome-related information of single cells are low throughput and high cost, requiring physical sorting and separate processes for imaging and sequencing, making it difficult to associate these types of information effectively.
Innovation Solution
A method involving compartments containing a single cell and beads linked to barcode nucleic acids, where nondestructive measurement information is associated with imaging information of the beads, and genome-related information is measured, allowing for integral detection using hybridized complexes and amplified products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If physical sorting and separate processes are used to obtain imaging and sequencing information, then measurement precision can be maintained, but productivity is low and cost is high
Solution Approach 1:
The patent merges imaging information and sequencing information detection into a single integrated process by co-encapsulating cells with imaging beads and sequencing beads in the same compartments. This allows both types of information to be obtained simultaneously from the same cell population, eliminating the need for separate physical sorting processes and thereby improving throughput while maintaining measurement precision.
Solution Approach 2:
The patent employs a universal compartment system that can simultaneously accommodate multiple bead types (imaging beads and sequencing beads) and perform multiple detection functions. This multi-functional approach allows a single experimental setup to generate both imaging and sequencing data, increasing productivity without sacrificing the precision of either measurement type.
2Reliability
If physical sorting is performed to associate imaging information with sequencing information, then information reliability can be ensured, but device complexity and operation difficulty increase
Solution Approach 1:
The patent uses compartment IDs as intermediary identifiers that link imaging information and sequencing information. Each compartment receives a unique ID that allows the system to associate the imaging data and sequencing data from the same compartment without requiring complex physical sorting operations. This intermediary approach ensures information reliability while simplifying the overall process.
Solution Approach 2:
The patent creates a digital copy of the compartment identification information that can be used to match imaging and sequencing data. Instead of physically sorting cells to associate data, the system uses copied identification codes to link the two types of information computationally, thereby reducing device complexity and operational difficulty while maintaining data reliability.
3Measurement precision
If separate processes are used for imaging and sequencing detection, then measurement precision is maintained, but loss of time increases
Solution Approach 1:
The patent enables continuous detection of both imaging and sequencing information within the same compartment system. By designing the compartments to contain both bead types and using a unified detection workflow, the system eliminates idle time between separate imaging and sequencing processes, thereby reducing total processing time while maintaining the detection accuracy of both measurements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables effective and integral detection of nondestructive and genome-related information of single cells, enhancing the association and value of these types of information without the need for physical sorting or high-cost processes.
Implementation Method 1
the second bead(s) is linked to a plurality of second barcode nucleic acids hybridizable with a genome-related nucleic acid
Implementation Method 2
imaging information of the first bead(s) in each compartment can be clearly distinguished from each other
Data Source
AI summary
The present invention provides a method for integrally detecting nondestructive measurement information and genome-related information of single cells.More specifically, the present invention uses a method including:preparing a plurality of compartments containing single cell or a derivative thereof, a first bead(s), and a second bead(s) per compartment;detecting both nondestructive measurement information of single cell and imaging information of the first bead(s) and associating the nondestructive measurement information of single cell with the imaging information of the first bead(s) before preparation of each compartment or in each compartment;obtaining a hybridized complex;producing an amplified product derived from the hybridized complex; andintegrally detecting nondestructive measurement information and genome-related information in single cell.