Single-Cell Microwell Cassette With Semipermeable Membrane Storage
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Solution Overview
Problem
Existing technologies fail to provide efficient solutions for the collection and storage of single cell samples prior to the creation of single-cell sequencing libraries, limiting asynchronous single-cell analysis.
Innovation Solution
A cassette system comprising a plunger assembly, membrane assembly, and loading assembly is developed to collect, store, and analyze bioparticles, enabling efficient asynchronous single-cell analysis by retaining bioparticles in microwells using a semi-permeable membrane and locking mechanism, with features like a semi-permeable membrane and microwell array for scalable and cost-effective processing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If droplets-based or microarray-based systems are used for single-cell analysis, then single-cell partitioning and analysis can be performed, but efficient collection and storage of single cell samples prior to library creation cannot be achieved
Solution Approach 1:
The patent implements preliminary action by enabling single cells to be captured and stored in microwells before library creation and sequencing analysis. The device allows cells to be isolated and preserved in a stable state, permitting asynchronous processing where sample collection occurs separately from downstream analysis, thus gaining time flexibility without compromising sample integrity
Solution Approach 2:
The patent introduces an intermediary storage system consisting of microwells with capture surfaces and sealing mechanisms. This intermediary structure holds single cells in a stable, preserved state between collection and analysis, acting as a buffer that decouples the timing of sample acquisition from library preparation and sequencing, thereby enabling asynchronous workflows
2Productivity
If centralized analytical processing is implemented, then processing efficiency and quality are improved, but device complexity increases
Solution Approach 1:
The patent applies segmentation by dividing the system into distinct functional modules: a capture module for single-cell isolation, a storage module with microwells for sample holding, and a processing module for centralized analysis. This modular segmentation allows centralized processing to be implemented efficiently while managing complexity through clear functional separation and standardized interfaces between modules
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The cassette system maintains the integrity and biological state of samples during storage and processing, facilitating centralized analytical processing and reducing variability, allowing for efficient and cost-effective single-cell interrogation.
Implementation Method 1
a semi-permeable membrane that comprises a membrane top surface and a membrane bottom surface
Data Source
AI summary
Disclosed herein are cellular cassettes for the storage, collection, and analysis of biological samples. The cellular cassette can enable easy sample collection and sealing of microwell arrays with semi-permeable membrane for stable storage and future processing of single cells. Also disclosed herein are systems and kits comprising one or more described cassettes. The described cassettes, systems, and kits can be used to create barcoded, single-cell sequencing libraries.Further described herein are methods of using the cassettes, systems, and kits.