Single-Label Target Nucleic Acid Detection at Different Temperatures
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional real-time detection methods for nucleic acid sequences require multiple labels or melting analysis to differentiate between multiple sequences, which are inefficient and time-consuming.
Innovation Solution
Detecting nucleic acid sequences using different detection temperatures with a single label in a single reaction vessel, employing signal-generating means that generate signals at specific temperatures to differentiate between sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional real-time detection methods use multiple labels to differentiate multiple target sequences, then detection accuracy is improved, but device complexity and cost increase
Solution Approach 1:
The patent changes the detection temperature parameter to differentiate between multiple target sequences. Each probe is designed with a specific melting temperature (Tm) that corresponds to its target sequence. By performing detection at different temperatures, the system can selectively detect different targets using the same fluorescent label, thus avoiding the need for multiple labels and reducing device complexity while maintaining detection accuracy
2Loss of substance
If melting analysis is used to detect multiple target sequences with a single label, then cost is reduced, but analysis time increases significantly
Solution Approach 1:
The patent implements periodic detection cycles at different temperatures rather than performing a complete melting curve analysis. The detection process cycles through different temperatures sequentially, detecting each target at its optimal temperature. This periodic approach maintains the cost advantage of single-label detection while dramatically reducing the total analysis time compared to traditional melting analysis
3Productivity
If multiple labels are used to detect multiple targets in parallel, then productivity is improved, but ease of operation deteriorates due to complex probe design
Solution Approach 1:
The patent makes the single fluorescent label universal for detecting multiple different target sequences. By designing probes with different melting temperatures that all use the same label, the system achieves multi-functionality. This allows parallel detection of multiple targets through temperature differentiation rather than requiring multiple labels, thereby improving ease of operation while maintaining productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient and cost-effective detection of multiple nucleic acid sequences with a single label, reducing analysis time and simplifying probe design.
Implementation Method 1
labeled probes or primers specifically hybridized with target nucleic acid sequences
Implementation Method 2
detect signals generated from fluorescent labels
Data Source
AI summary
Disclosed are detections of target nucleic acid sequences using different detection temperatures. Employing different detection temperatures enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel. The conventional technologies detect a plurality of target nucleic acid sequences by a melting analysis after target amplification. Unlikely, the detection does not require a melting analysis after target amplification, such that the time for analysis is greatly reduced.