Single-Signal Multi-Analyte Detection Bridging Complex
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Solution Overview
Problem
Current methods for detecting multiple analytes require multiple signals or reactions, making interpretation complex and challenging, especially when adulterants have multiple detectable characteristics, necessitating a method for simplified detection with a single signal.
Innovation Solution
A complex comprising a capture reagent affixed to a solid support, analytes with interaction units, and a signal detection unit that binds to form a bridging complex, allowing concurrent detection of multiple analytes with a single signal, using heterologous interaction units and multivalent capture reagents like immunoglobulins or biotin.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple signals or multiple reactions are used to detect multiple analytes, then detection capability is improved, but interpretation complexity increases
Solution Approach 1:
The patent combines multiple analyte detection capabilities into a single reaction system where multiple analytes are detected simultaneously using a single signal. The bridging complex merges the detection of first and second analytes through shared interaction units and capture reagents, eliminating the need for separate reactions and signals for each analyte.
Solution Approach 2:
The patent implements multi-functionality through interaction units that can bind to multiple different capture reagents. A single analyte with multiple interaction units can be detected by different capture reagents targeting the same analyte, allowing one detection system to handle multiple analytes through universal binding mechanisms.
2Measurement precision
If multiple capture reagents targeting the same analyte are used, then detection specificity is improved, but false positives increase
Solution Approach 1:
The patent introduces a bridging complex as an intermediary that connects multiple capture reagents to multiple analytes through interaction units. This bridging structure ensures that signal generation only occurs when the complete complex forms, requiring simultaneous presence of all targeted analytes, thereby eliminating false positives from individual capture reagent bindings.
Solution Approach 2:
The patent applies local quality by making each interaction unit specific to particular capture reagents while maintaining the ability to form a complete bridging complex only when all components are present. The signal detection unit is localized to detect only the complete bridging complex, ensuring high specificity without false positives from partial bindings.
3Reliability
If a bridging complex with multiple capture reagents is used, then false positives are reduced, but system complexity increases
Solution Approach 1:
The patent employs preliminary action by pre-attaching capture reagents to the solid support and pre-forming the bridging complex structure with interaction units designed to bind specific capture reagents. This preliminary preparation ensures that when analytes are introduced, the complete complex forms automatically without requiring complex real-time control mechanisms, reducing false positives while maintaining system simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the simultaneous detection of multiple analytes with a single signal, simplifying interpretation and improving detection accuracy by forming a bridging complex that only generates a detectable signal when all analytes are present, reducing false positives and enhancing specificity.
Implementation Method 1
a first capture reagent affixed to a solid support... wherein the first capture reagent binds to the first interaction unit of the first analyte of interest
Implementation Method 2
the bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the first analyte and the first interaction unit of the second analyte of interest
Implementation Method 3
the signal detection unit comprises a capture reagent that binds to the second interaction unit of the second analyte of interest
Data Source
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AI summary
Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.