Singular Optics Superresolution for Multi-Fluorophore Localization
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Solution Overview
Problem
Existing superresolution fluorescence microscopy techniques face limitations in achieving both high lateral and longitudinal resolution, require high light intensities harmful to cells, are complex to operate, and struggle to detect multiple fluorophores in the same volume, lacking the simplicity and affordability of standard microscopes.
Innovation Solution
A method and device using a sequence of compact light distributions of different topological families, generated by interference between regular and singular waves, to determine the spatial position of fluorophores, allowing for high-precision measurement and recognition of multiple fluorophores in the same illuminated volume, utilizing optical modules for lateral and longitudinal superresolution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescence microscopy is used, then the system is simple and affordable, but the spatial resolution is limited by the optical diffraction limit (cannot resolve details smaller than 200 nm)
Solution Approach 1:
The patent changes the topological parameter of light distributions by using sequences of compact light distributions with different topological families (different winding numbers). This allows superresolution measurement without requiring complex hardware modifications, resolving the contradiction between improved measurement precision and device complexity
Solution Approach 2:
The patent replaces complex mechanical superresolution systems with a computational approach using singular optics and topological analysis of light distributions. The superresolution is achieved through algorithmic processing of intensity patterns rather than complex optical mechanical arrangements
2Measurement precision
If high light intensity is used to achieve superresolution, then the spatial resolution improves, but the light intensity becomes harmful to cells
Solution Approach 1:
The patent uses periodic sequences of compact light distributions with different topologies to illuminate fluorophores. This periodic illumination pattern allows superresolution measurement while distributing the light dose over time, reducing peak intensity and phototoxicity to cells
Solution Approach 2:
The patent changes the topological parameters of light distributions rather than increasing intensity. By using different topological families (different winding numbers) in the light distribution sequence, superresolution is achieved through topological differentiation rather than intensity enhancement, avoiding cellular damage
3Measurement precision
If conventional microscopy is used, then the operation is simple, but the ability to detect multiple fluorophores in the same volume is limited
Solution Approach 1:
The patent uses topological parameters (winding numbers) of light distributions as distinguishing features for different fluorophores. Each fluorophore responds differently to light distributions with different topologies, enabling simultaneous detection and differentiation of multiple fluorophores in the same volume through their unique topological response patterns
Solution Approach 2:
The patent introduces topological analysis as an intermediary between light illumination and fluorophore detection. The topological features of light distributions serve as mediators that encode spatial and identification information, allowing multiple fluorophores to be distinguished through their differential responses to topologically varied illumination
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-precision measurement of fluorophore attributes and simultaneous detection of multiple fluorophores with reduced light intensity, improving spatial resolution beyond the optical diffraction limit and enhancing operational simplicity and cost-effectiveness.
Implementation Method 1
generated by interference between regular and singular waves
Implementation Method 2
detecting the fluorescence emitted by the fluorophores
Data Source
AI summary
A method of optical measurement for determining a spatial position of at least one luminous object in a sample includes: projecting onto the sample a dynamically optimized sequence of compact luminous distributions of different topological families; wherein the dynamically optimized sequence is determined based on data selected from the group consisting of a positioning hypothesis and a first set of measures; for each compact luminous distribution in the optimized sequence, generating an image of the at least one luminous object as illuminated thereby; and algorithmically analyzing the generated images to obtain spatial position information of the at least one luminous object.