In Vitro Screening for Skin Hydration and Anti-Aging Compounds

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Solution Overview

Problem

Current methods fail to effectively prevent or attenuate skin aging, particularly photo-aging, and hydration, as they do not adequately address the decrease in gene expression of LARGE, HS6ST2, and ST8SIA1, which are crucial for glycosylation and skin health, especially under UVB irradiation.

Innovation Solution

An in vitro method for screening compounds that activates the expression of LARGE, HS6ST2, and ST8SIA1 genes in keratinocytes by measuring mRNA or protein levels, identifying compounds that increase expression by at least 1.4 or 1.6 fold, which can be used to develop cosmetic compositions for skin care.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional methods are used for skin care, then general skin maintenance is achieved, but they fail to prevent or attenuate skin aging and dehydration effectively

Engineering Contradiction:

Improveeffectiveness in preventing skin agingVSAvoidactivation of gene expression

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The invention changes the molecular parameter by introducing compounds that specifically activate gene expression (LARGE, HS6ST2, ST8SIA1) to enhance glycosylation. This parameter change transforms the biochemical environment to improve skin aging prevention and hydration, moving from conventional general maintenance to targeted molecular intervention.

Inventive Principle:

Principle #35Parameter changes

2Object-affected harmful factors

If UVB irradiation occurs, then skin cells are exposed to environmental stress, but gene expression of LARGE, HS6ST2, and ST8SIA1 decreases leading to abnormal glycosylation

Engineering Contradiction:

ImproveUV-induced gene expression decreaseVSAvoidglycosylation normality

Core Design Contradiction:

Object-affected harmful factorsVSStability of the object's composition

Solution Approach 1:

The invention applies preliminary anti-action by using compounds to pre-activate gene expression before UVB damage can fully occur, or to counteract the expression decrease immediately upon UV exposure. This prevents the downstream effect of abnormal glycosylation by maintaining adequate levels of LARGE, HS6ST2, and ST8SIA1 expression despite UV stress.

Inventive Principle:

Principle #9Preliminary anti-action

Solution Approach 2:

The invention converts the harmful effect of UVB-induced gene suppression into a beneficial target for intervention. By identifying and treating the specific genes (LARGE, HS6ST2, ST8SIA1) that decrease under UV stress, the invention transforms UV damage response into an opportunity for targeted protection and repair through compound administration.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

3Reliability

If gene expression of LARGE, HS6ST2, and ST8SIA1 is not activated, then skin hydration and cell communication are compromised, but conventional treatments do not address this specific mechanism

Engineering Contradiction:

Improveskin hydration and cell communicationVSAvoidspecificity of treatment mechanism

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The invention applies local quality by specifically targeting the glycosylation pathway through compounds that activate particular genes (LARGE, HS6ST2, ST8SIA1) rather than using general skin care approaches. This localized molecular intervention ensures precise action on the biochemical mechanisms responsible for skin hydration and cell communication, achieving reliable results through specificity.

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The identified compounds significantly enhance skin hydration and prevent aging by correcting abnormal glycosylation and improving skin cell communication and function, effectively addressing UV-induced senescence and dehydration.

Implementation Method 1

activates the expression of at least one gene selected from LARGE, HS6ST2 and ST8SIA1

Methodology Applied

Scientific EffectGene expression activation:

Implementation Method 2

genes encoding proteins involved in post-translational modifications, particularly in glycan synthesis, comprise glycosyltransferases

Methodology Applied

Scientific EffectGlycosylation: Enzyme

Data Source

PatentUS9745629B2In vitro method for screening for candidate compounds for preventing and/or attenuating ageing of the skin, and/or for hydrating the skin

Publication Date: 2017.08.29 CHANEL PARFUMS BEAUTE SAS

AI summary

The identification and the use of compounds which activate the expression of at least one gene selected from LARGE, HS6ST2 and ST8SIA1 is provided. An in vitro method for screening for candidate compounds includes:(a) bringing at least one test compound in contact with a sample of keratinocytes in vitro;(b) measuring the expression of at least one gene selected from LARGE, HS6ST2 and ST8SIA1, in the keratinocytes; and(c) selecting the compounds for which an activation of at least 1.4 fold of the expression of at least one of the LARGE, HS6ST2 and ST8SIA1 genes is measured in the keratinocytes treated in (a) compared with the untreated keratinocytes.The candidate compounds may be useful for preventing and/or attenuating ageing, and/or for hydrating skin.