Ex Vivo Skin Tissue Model With Porous Layer for Long-Term Viability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current skin tissue analysis methods lack data consistency and longevity, hindering the provision of clinically relevant endpoints, and animal and human trials are costly and risky.
Innovation Solution
An ex vivo skin tissue system with a porous layer and adipose layer to promote mass transport of reagents and fluids, supporting skin tissue viability for at least one week, and includes features like a gel layer, cell composition, and channels for perfusion and monitoring.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional skin tissue analysis methods are used, then the analysis can be performed, but the data consistency and tissue longevity are insufficient
Solution Approach 1:
The patent employs a porous layer with gyroid structures to enhance mass transport of reagents and nutrients into the skin tissue sample. The porous architecture increases surface area and facilitates efficient diffusion, thereby maintaining tissue viability and physiological function over extended periods, directly addressing the tissue longevity issue while ensuring consistent data quality
Solution Approach 2:
The system integrates multiple components including skin tissue samples, adipose layers, porous layers with gyroid structures, and gel layers into a composite ex vivo model. This composite structure mimics the complex architecture of human skin, improving physiological relevance and data consistency while extending tissue survival through optimized nutrient and oxygen delivery
2Reliability
If animal and human trials are conducted, then clinically relevant endpoints can be obtained, but the costs and experimental risks are high
Solution Approach 1:
The patent creates ex vivo skin tissue models that replicate the structure and function of in vivo human skin. By culturing human skin tissue samples ex vivo with optimized mass transport, the system produces clinically relevant endpoints without requiring animal or human trials, thereby reducing experimental complexity and ethical concerns while maintaining reliability
Solution Approach 2:
The ex vivo skin tissue model serves as an intermediary system between in vitro cell cultures and in vivo animal/human trials. This intermediate model provides clinically relevant data with reduced complexity by using human-derived tissue that maintains physiological relevance without the full complexity and ethical constraints of living organism trials
3Duration of action of stationary object
If skin tissue samples are cultured ex vivo, then the tissue viability can be extended, but the mass transport of reagents into the tissue is limited
Solution Approach 1:
The porous layer with gyroid structures provides enhanced mass transport pathways for reagents, nutrients, and oxygen to penetrate deep into the skin tissue sample. The three-dimensional porous architecture increases the effective surface area and creates efficient diffusion channels, ensuring adequate substance delivery to maintain extended tissue viability
Solution Approach 2:
The gyroid structures in the porous layer create a three-dimensional network that facilitates multi-directional mass transport. This three-dimensional architecture allows reagents to reach tissue cells more effectively compared to traditional two-dimensional culture surfaces, thereby supporting extended tissue viability through improved substance distribution
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system provides reliable, long-term viability and analysis of skin tissues, enabling clinically relevant endpoint assessment and reducing the need for animal trials.
Implementation Method 1
the porous layer includes an arrangement relative to the skin tissue sample so as to promote mass transport of reagents and/or fluid into the skin tissue sample
Implementation Method 2
the porous layer includes an arrangement relative to the skin tissue sample so as to promote mass transport of reagents and/or fluid into the skin tissue sample
Implementation Method 3
at least one pore of the porous layer includes a gyroid
Data Source
AI summary
This disclosure relates to skin tissue systems, devices, and methods. Systems described include ones comprising: a tissue sample; an insert; and a porous layer configured to promote mass transport into the tissue sample; wherein the skin tissue system is capable of supporting tissue viability for at least one week. Methods described include ones for skin tissue analysis comprising: contacting a skin tissue array with at least one test agent; measuring a plurality of datasets following the contacting; mapping the plurality of datasets based on a scoring panel result; and generating at least one threshold criteria for selecting the therapeutic agent from the at least one test agent based on the mapped datasets. Such innovations described herein provide for an ex vivo model tissue system and use of the model tissue system for analytical activities such as screening of nutrients, pharmaceutical activity, and toxicity in tissue samples.