SKP1 Polypeptide Overexpression in Recombinant Microalgae
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Solution Overview
Problem
Microalgae biomass productivity is low, leading to high costs for algal biofuels and products, necessitating improvements in yield.
Innovation Solution
Overexpression of specific genes associated with the SCF complex, such as SKP1 polypeptides, in microorganisms like microalgae to enhance biomass and lipid productivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microalgal culture methods are used, then production costs are high, but biomass productivity remains low
Solution Approach 1:
The patent applies parameter changes by modifying the expression level of cell cycle-regulating proteins through genetic engineering. Specifically, it involves overexpressing or downregulating proteins such as cyclins, CDKs, and SCF complex components to accelerate cell cycle progression and improve biomass productivity, thereby resolving the contradiction between low productivity and high production cost
2Quantity of substance
If biomass yield is increased, then production cost decreases, but cell cycle regulation becomes more complex
Solution Approach 1:
The patent applies segmentation by dividing the complex cell cycle regulation into modular protein complexes (such as SCF complex with SKP1, CUL1, and F-box components) that can be independently manipulated. This allows targeted modification of specific regulatory pathways to enhance biomass yield while managing the complexity through structured, compartmentalized protein interactions
Data Source
Figure 1
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Figure 2C~2E
AI summary
The application provides recombinant microorganisms with increased productivity with respect to control or wildtype microorganisms. The recombinant microorganisms can include a non-native gene encoding a SKPl polypeptide or a CHORD-derived polypeptide. Increased productivity can be increased biomass or lipid productivity. These recombinant microorganisms can be used to produce products of interest.