Decellularization Reactor With Twin Vessels for Small Tissue Samples
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Solution Overview
Problem
Existing technologies face challenges in efficiently decellularizing small quantities of various types of xenogeneic organ tissues, particularly those like the cornea of a pig, which are difficult to process using large-scale facilities, and there is a need for a device that can efficiently decellularize these tissues while allowing for comparison of their physical properties.
Innovation Solution
A decellularization reaction apparatus comprising a first and second reaction vessel, mesh chambers, solution circulation parts, and ex-situ analysis devices that allow for the decellularization process of small quantities of xenogeneic organs, enabling efficient stirring, solution circulation, and physical property analysis of the decellularization solutions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a large-scale reactor is used for decellularization, then mass production capability is improved, but the ability to process small quantities of xenogeneic organ tissue deteriorates
Solution Approach 1:
The reactor is divided into multiple independent reaction vessels (first reaction vessel and second reaction vessel), each capable of processing small quantities of tissue separately. This segmentation allows the system to handle small samples effectively while maintaining the infrastructure for scalable processing.
Solution Approach 2:
The reaction vessels are designed with universal functionality to accommodate different types of xenogeneic organ tissues (cornea, heart, etc.) and process them through the same decellularization protocol. The mesh chambers and circulation systems can handle various tissue types and quantities flexibly.
2Quantity of substance
If a large-scale facility is used for decellularization, then processing capacity is improved, but the ease of operation for small samples deteriorates
Solution Approach 1:
By dividing the large-scale facility into multiple smaller, independent reaction vessels, each vessel becomes easier to operate and manage. The mesh chambers can be individually accessed, cleaned, and maintained, simplifying operations for small sample processing while the overall system maintains high processing capacity through parallel operations.
3Adaptability or versatility
If detailed tissues of various xenogeneic organs are processed, then versatility is improved, but device complexity increases
Solution Approach 1:
The reaction vessels are designed with universal components including mesh chambers that can accommodate different tissue types, circulation systems that handle various fluids, and heating/cooling systems that work across different processing protocols. This universality enables the system to process detailed tissues from various xenogeneic organs without requiring separate specialized equipment for each tissue type.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus effectively decellularizes small quantities of xenogeneic organs and allows for efficient evaluation of their physical properties, facilitating uniform stirring and analysis of decellularization solutions through twin reaction vessel and mesh chamber systems.
Implementation Method 1
a first driving part for rotating the first mesh chamber to be stirred within the first reaction vessel
Implementation Method 2
a first solution circulation part connected in a way that allows communication between the first reaction vessel and a first cover that covers the first reaction vessel and intended to circulate a first decellularization solution to the first reaction vessel and the first cover
Implementation Method 3
The first outer tube may be equipped with a first refrigerant injection port for injecting refrigerant into the space between the first outer tube and the first inner tube, and a first refrigerant discharge port for discharging the injected refrigerant to the outside of the first outer tube
Data Source
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AI summary
The decellularization reaction apparatus is provided. The decellularization reaction apparatus according to the present invention comprises a first reaction vessel for storing a first decellularization solution for performing a decellularization reaction, a first ex-situ analysis application device for extracting a tissue of a first xenogeneic organ and a first decellularization solution undergoing a decellularization reaction within the first reaction vessel and measuring the physical property of the first decellularization solution ex-situ.