Smartphone BNP Aptamer Fluorescence Detection Device
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Solution Overview
Problem
Current methods for detecting brain natriuretic peptide (BNP) concentrations in blood, such as ELISA and CLIA, are expensive, complex, and suffer from low sensitivity and cross-reaction issues, limiting their effectiveness and application in clinical settings.
Innovation Solution
A smartphone-based BNP aptamer fluorescence detection device that integrates an aptamer fluorescence sensor and a detection control mechanism, utilizing a direct plug-in power supply from a smartphone to power a fluorescence rapid detection mechanism, which captures and processes fluorescence signals to accurately determine BNP concentrations without complex sample pre-treatment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If ELISA method is used for BNP detection, then specificity is improved, but sensitivity deteriorates and accuracy becomes low
Solution Approach 1:
The patent changes the detection method from enzyme-based colorimetric detection (ELISA) to fluorescence-based detection using aptamers. This parameter change in detection technology enables high sensitivity through fluorescence signal amplification while maintaining high specificity through aptamer-antigen binding, simultaneously resolving both the sensitivity and specificity requirements.
Solution Approach 2:
The patent replaces the mechanical/enzymatic detection system of ELISA with an optical detection system based on fluorescence. The aptamer-fluorescein conjugate system uses optical signals instead of enzymatic reactions, enabling more sensitive detection while eliminating the cross-reaction problems that limit ELISA accuracy.
2Measurement precision
If CLIA commercial detection platform is used, then sensitivity is improved and detection range is wide, but cost increases and anti-interference ability weakens
Solution Approach 1:
The patent uses disposable aptamer-fluorescein conjugate probes that are simple to manufacture and can be produced at low cost. These single-use probes eliminate the need for expensive commercial CLIA platforms while maintaining high sensitivity through fluorescence detection, providing a cost-effective alternative for BNP measurement.
Solution Approach 2:
The patent extracts the essential detection function from complex commercial CLIA systems by using only the core aptamer-probe binding mechanism combined with fluorescence detection. This extraction eliminates unnecessary complexity and cost while preserving the high sensitivity advantage, creating a simplified detection system suitable for point-of-care use.
3Ease of operation
If aptamer fluorescence sensor with smartphone integration is used, then portability and cost-effectiveness are improved, but detection sensitivity must be maintained
Solution Approach 1:
The patent makes the smartphone serve multiple functions: power supply for the excitation light source, processor for analyzing fluorescence signals, and display device for showing results. This multi-functionality enables the system to maintain high detection sensitivity while being portable and cost-effective, as the smartphone replaces multiple separate components.
Solution Approach 2:
The patent merges the detection mechanism, power supply, signal processing, and result display into a single integrated system using the smartphone as the central hub. This combination maintains detection sensitivity through proper optical design while achieving portability and cost-effectiveness by eliminating the need for separate expensive components.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device provides a portable, cost-effective, and highly sensitive BNP detection method, capable of real-time detection with high specificity and rapid results, overcoming the limitations of traditional methods by using oligonucleotides as aptamers and carboxylated graphene oxide as a fluorescence quencher, reducing cross-reactions and improving detection efficiency.
Implementation Method 1
the excitation light source is configured to turn on and off according to the control signal of the microcontroller, and generate excitation light with a set wavelength
Implementation Method 2
the signal acquisition and photoelectric conversion module is configured to capture a fluorescence signal generated form the solution to be detected
Implementation Method 3
the signal acquisition and photoelectric conversion module is configured to capture a fluorescence signal generated form the solution to be detected according to the control signal of the microcontroller, and convert the fluorescence signal into an electrical signal
Data Source
AI summary
The present invention discloses a BNP aptamer fluorescence detection device based on a smart phone and a sensing method thereof, including a detection control mechanism, a fluorescence rapid detection mechanism and an aptamer fluorescence sensor. The aptamer fluorescence sensor uses oligonucleotides marked by carboxy fluorescein as an aptamer to capture the BNP specificity. The detection control mechanism receives a fluorescence signal to display test results. The fluorescence rapid detection mechanism uses the OTG function as a plug-in power supply. The present invention fills the gap of portable fluorescence detection for digital BNP.