snoRNA Markers for De-Servitization State Detection and Regulation
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Solution Overview
Problem
There is a lack of effective molecular markers to define and regulate the servitization and de-servitization states of cells, which are associated with various physiological and disease conditions.
Innovation Solution
The use of small nucleolar RNA (snoRNA) as a detection marker and regulatory agent to identify and switch between enmunting and demunting states in cells, utilizing snoRNA markers such as SNORA41, SNORD85, SNORD115, SNORD116, SNORD14c, SNORD14d, SNORD55, SNORD15b, SNORD23, SNORD96a, SNORD123, SNORD34, and SNORD82, through methods like RT-PCR, real-time PCR, Northern blotting, high-throughput sequencing, and CRISPR/Cas9 regulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional detection methods are used to identify cell servitization state, then detection capability is limited, but the complexity of the system increases due to lack of specific markers
Solution Approach 1:
The patent extracts and identifies specific snoRNA molecules (SNORA41, SNORD85, SNORD115, SNORD116, SNORD14c, SNORD14d, SNORD55, SNORD15b, SNORD23, SNORD96a, SNORD123, SNORD34, SNORD82) as detection markers for cell servitization state. By isolating these specific molecular markers from the complex cellular environment, the patent enables precise detection of cell state without requiring complex analytical systems.
2Difficulty of detecting and measuring
If no specific molecular markers are available, then the difficulty of detecting cell state increases, but the time required for diagnosis and treatment is extended
Solution Approach 1:
The patent performs preliminary identification and characterization of snoRNA markers that specifically indicate cell servitization state. By establishing these markers in advance through research and validation, the patent enables rapid clinical detection and diagnosis without requiring time-consuming exploratory analysis when clinical decisions are needed.
3Reliability
If conventional therapy approaches are used, then treatment effectiveness is limited, but the inability to regulate cell state specifically prevents therapeutic intervention
Solution Approach 1:
The patent establishes a feedback mechanism where snoRNA expression levels serve as indicators of cell servitization state, and therapeutic interventions are designed to modulate these snoRNA levels. By monitoring snoRNA expression and adjusting treatment accordingly, the patent enables adaptive therapeutic regulation that responds to actual cell state, improving treatment effectiveness and versatility.
Data Source
AI summary
The present invention provides a use for a small nucleolar RNA (snoRNA) or a detection reagent thereof, for use in detection of a servitization state of cells; moreover, detection and regulating methods for the snoRNA are established.