SNP Loci Combination for Wool Fiber Diameter Analysis
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Solution Overview
Problem
Current sheep SNP research lacks studies on specific traits, particularly combinations of SNP loci associated with wool fiber diameter in fine wool sheep.
Innovation Solution
The disclosure provides a combination of 33 SNP loci associated with wool fiber diameter in fine wool sheep, determined through high-depth whole-genome resequencing data of four representative Chinese fine wool sheep breeds, using the Ovis aries reference genome version 4.0 as a reference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional breeding methods are used to select for wool fiber diameter, then breeding accuracy is limited, but the breeding process is simpler and less costly
Solution Approach 1:
The patent replaces traditional mechanical/phenotypic selection methods with molecular genetic marker analysis. By using SNP markers associated with wool fiber diameter and implementing genome-wide association studies, the invention substitutes direct physical measurement and visual assessment with DNA-based detection, achieving superior breeding accuracy through genetic information rather than mechanical evaluation of wool traits
Solution Approach 2:
The patent introduces molecular genetic markers as an intermediary between phenotype and genotype. These markers serve as mediators that link observable wool fiber diameter traits to underlying genetic variations, enabling indirect selection through marker analysis rather than direct phenotypic evaluation, thus improving selection accuracy while providing a systematic framework for breeding programs
2Measurement precision
If genome-wide association studies with multiple SNP loci are implemented, then detection accuracy of wool fiber diameter improves, but detection cost and complexity increase
Solution Approach 1:
The patent segments the genome into specific chromosomal regions and identifies discrete SNP marker loci associated with wool fiber diameter. By dividing the complex genomic information into manageable segments (specific SNP positions on different chromosomes) and analyzing them individually through targeted assays, the invention achieves high detection accuracy while reducing the practical complexity compared to analyzing the entire genome simultaneously
Solution Approach 2:
The patent focuses detection efforts on specific local regions of the genome where SNP markers are known to be associated with wool fiber diameter traits. Rather than uniformly analyzing all genomic regions, the invention concentrates resources on identifying and detecting variations at specific chromosomal positions (e.g., chr 1 position 203825947, chr 5 position 93335425), thereby achieving high detection accuracy with reduced overall complexity
3Productivity
If traditional phenotypic selection is used, then breeding cost is lower, but breeding efficiency and speed are reduced
Solution Approach 1:
The patent performs preliminary genetic analysis by identifying and validating SNP markers associated with wool fiber diameter before implementing breeding programs. By conducting genome-wide association studies and establishing marker-trait relationships in advance, the invention enables early-stage genetic assessment of breeding stock, allowing producers to make informed selection decisions before investing in actual breeding operations, thus improving overall breeding efficiency
Solution Approach 2:
The patent replaces time-consuming phenotypic evaluation and multi-generation breeding trials with rapid DNA-based genotyping. By substituting mechanical measurement of wool traits and long-term performance monitoring with molecular marker analysis, the invention accelerates the breeding cycle and improves productivity, making the breeding process more efficient despite increased per-sample detection costs
Data Source
AI summary
The present disclosure pertains to the biological field, specifically to a combination of 33 single nucleotide polymorphism (SNP) loci associated with the wool fiber diameter of fine wool sheep, determined using Ovis aries reference genome version 4.0. It also covers the use of reagents for detecting genotypes of these SNP loci in wool fiber diameter analysis or molecular marker-assisted selection of fine wool sheep. Additionally, it includes molecular probe combinations, gene chips, and kits based on the SNP loci. These tools facilitate early-stage wool fiber trait analysis, enabling individual selection, reducing generation intervals, accelerating breeding processes, and lowering costs. This disclosure provides crucial support for the identification, breed preservation, and molecular genetic breeding of fine wool sheep.