SNP Molecular Marker Combination for AA Broiler Identification
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Solution Overview
Problem
Current methods for identifying AA broilers rely on traditional morphological characteristics, which are subjective and unreliable, especially for chicks or meat products, leading to a need for accurate genetic-level identification.
Innovation Solution
A SNP molecular marker combination comprising 25 distinct SNP sites, optimized through whole genome resequencing and comparison with other chicken breeds, is developed to specifically identify AA broilers, along with a detection kit using a KASP primer set for rapid and accurate genotyping.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional morphological identification methods are used, then the identification process is simple, but the accuracy and reliability are low
Solution Approach 1:
The patent replaces traditional mechanical/morphological identification methods with molecular genetic detection methods. Specifically, it uses SNP marker detection through PCR amplification and fluorescence detection to identify AA broiler chickens at the DNA level, substituting subjective visual assessment with objective molecular biology techniques, thereby significantly improving identification accuracy.
Solution Approach 2:
The patent introduces SNP molecular markers as intermediaries between the target object (AA broiler chicken DNA) and the detection system. These markers serve as specific genetic signatures that mediate the identification process, allowing accurate distinction of AA broilers from other breeds through detection of characteristic nucleotide variations at specific genomic positions.
2Adaptability or versatility
If morphological characteristics are used for identification, then no special equipment is needed, but identification is unreliable for chicks and meat products
Solution Approach 1:
The patent replaces morphological observation with molecular genetic detection that works uniformly across all sample types. By extracting DNA from any source (chick blood, feather roots, or meat product samples) and detecting SNP markers through PCR and fluorescence, the method achieves consistent reliability regardless of sample age or form, overcoming the limitations of appearance-based identification.
3Measurement precision
If SNP molecular marker combination is used, then identification accuracy is high, but detection cost and complexity increase
Solution Approach 1:
The patent segments the identification task into detection of specific SNP markers at defined genomic positions. Rather than analyzing the entire genome or relying on multiple morphological traits, it focuses on detecting variations at specific nucleotide positions (e.g., position 51 of reference sequences), breaking down the complex identification problem into discrete, detectable molecular events that can be processed through standardized PCR and fluorescence detection protocols.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The SNP molecular marker combination achieves a high accuracy rate of 99.47% in identifying AA broilers, providing a reliable method for genetic breed identification and traceability, thereby addressing the limitations of morphological identification.
Implementation Method 1
The detection kit includes a polymerase chain reaction (PCR) primer set for detecting genotypes of the SNP molecular marker combination
Data Source
AI summary
A SNP molecular marker combination for identifying an AA broiler includes 25 SNP molecular markers, SNP sites of the SNP molecular markers 1 to 25 are located at a 51st position of each of nucleotide sequences shown in SEQ ID NO: 1 to 25. The SNP molecular marker combination for identifying the AA broiler in the disclosure has apparent species specificity of the AA broiler, is a unique mutation and importance determining site for the AA broiler, and can quickly identify authenticity of the AA broiler with less genotype information, providing new technical references for the identification, conservation, and genetic breeding of chicken breeds in the future.