Sodium Acetate Storage Medium for Adipose Cell Viability
Solution Overview
Problem
Current liposuction techniques, particularly using Klein's tumescent solution, result in unpredictable resorption rates and low graft survival due to ischemia and cytotoxic effects on adipose tissue-derived stem cells (ASCs), limiting the viability and survival of ASCs during and after transplantation.
Innovation Solution
A composition comprising adipose tissue and/or cells derived thereof, combined with sodium acetate, which acts as a storage medium to significantly prolong the viability and survival of ASCs, including CD34+ ASCs, by maintaining higher cell counts and reducing cytotoxic effects, thereby enhancing the chances of successful tissue or cell transplantations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If Klein's tumescent solution is used for liposuction, then the procedure can be performed under local anesthesia with minimized blood loss, but the ASCs suffer cytotoxic effects and graft survival decreases
Solution Approach 1:
The patent removes the cytotoxic components (lidocaine and epinephrine) from the tumescent solution while retaining the beneficial effects of tumescent liposuction. This extraction of harmful elements allows the procedure to be performed safely without compromising ASC viability or graft survival.
Solution Approach 2:
The patent fundamentally changes the chemical composition parameters of the tumescent solution by eliminating lidocaine and epinephrine. This parameter change transforms the solution from cytotoxic to cytocompatible, allowing it to preserve ASC viability while still enabling tumescent liposuction under local anesthesia.
2Loss of time
If lipoaspirate is stored for extended periods, then transport and processing time is increased, but ASC viability decreases due to ischemia
Solution Approach 1:
The patent applies preliminary protective action by coating ASCs with hyaluronic acid before storage and transport. This pre-treatment creates a protective environment that maintains ASC viability during extended storage periods, effectively decoupling storage time from viability loss.
Solution Approach 2:
Hyaluronic acid serves as an intermediary substance between the ASCs and the storage environment. It mediates the interaction by providing a protective matrix that maintains cell viability, allowing extended storage without direct harmful exposure to the storage conditions.
3Duration of action of moving object
If lidocaine and epinephrine are included in the tumescent solution, then anesthesia duration is prolonged and blood loss is minimized, but ASC cytotoxicity increases
Solution Approach 1:
The patent converts the harmful cytotoxic effects of lidocaine and epinephrine into beneficial outcomes by completely removing these substances. This eliminates the harm to ASCs while alternative methods are used to achieve prolonged anesthesia and minimized blood loss through other means that are cytocompatible.
Data Source
AI summary
The present invention relates to the field of medicine, in particular to internal and aesthetic regenerative medicine. More specifically, the present invention relates to a composition suitable for use as a transport medium for adipose tissue and/or cells derived thereof. The invention further relates to a liposuction infiltration formulation and a process to obtain lipoaspirate material.